AP61497 | Anti-Histone H3 (MonoMethyl K26) Antibody Clinisciences

Western blot analysis of Histone H3 (MonoMethyl K26) expression in A549 (A), H1688 (B) whole cell lysates.
Immunohistochemical analysis of Histone H3 (MonoMethyl K26) staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Direct ELISA antibody dose-response curve using Anti-Histone H3 (MonoMethyl K26) Antibody. Antigen (methyl-peptide and non-methyl-peptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immuno electron microscopy EIA=Enzyme Immunoassay	WB, IHC
Primary Accession	P68431, Q71DI3, P84243, Q6NXT2
Reactivity	Human, Mouse, Rat, Zebrafish, Chicken, Bovine
Host	Rabbit
Clonality	Polyclonal

Additional Information
Other Names	HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; HIST2H3A; HIST2H3C; H3F2; H3FM; HIST2H3D; Histone H3.2; Histone H3/m; Histone H3/o; H3F3A; H3.3A; H3F3; PP781; H3F3B; H3.3B; Histone H3.3; H3F3C; Histone H3.3C; Histone H3.5
Target/Specificity	Recognizes endogenous levels of Histone H3 with a site at MonoMethyl K26 protein.
Dilution	WB~~WB (1/500 - 1/1000), IH (1/50 - 1/200) E~~WB (1/500 - 1/1000), IH (1/50 - 1/200)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

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Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

Background

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Histone H3 with a site at MonoMethyl K26. The exact sequence is proprietary.

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Cat# AP61497

Anti-Histone H3 (MonoMethyl K26) Antibody

Cat# AP61497

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Brand : Abcepta

Contact local distributor :

Background

Anti-Histone H3 (MonoMethyl K26) Antibody

Cat# AP61497

Contact local distributor :

Brand : Abcepta

Contact local distributor :

Background

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