Anti-IAA - Indole-3-acetic acid (C1') (1mg)
Cat# AS09420
Size : 1mg
Brand : Agrisera
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Phone : +1 850 650 7790
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AS09 420 | Clonality: Polyclonal | Host: Rabbit
- Product Info
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Immunogen: BSA-conjugated, via C1 carboxyl group, indole-3-acetic acid (C1')
Host: Rabbit Clonality: Polyclonal Purity: Total IgG. Protein G purified in PBS. Format: Lyophilized Quantity: 1 mg Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: ELISA (ELISA), Radioimmunoassay (RIA) Recommended dilution: 2-10 µl/15 ml; Specific information about dilution is going to be included on the vial - Reactivity
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Confirmed reactivity: IAA | indole-3-acetic acid (C1') Predicted reactivity: IAA | indole-3-acetic acid (C1') Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Additional Information
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Additional information: IAA | Indole-3-acetic acid (C1') (1mg) - Background
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Background: Indole 3 acetic acid (IAA) is the principal auxin in higher plants. This hormone is produced in in cells in the apex and young leaves of a plant. Plant cells synthesize IAA from tryptophan. Different effects caused by auxines include: induction of cell elongation and cell division and have a subsequent results for plant growth and development.
- Protocols
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Molar cross-reactivities of different auxins and related compounds with anti-IAA (C1) antibodies.
Presented data are expressed as percentage ratio of molar concentrations of appropriate auxin and competitor producing 50% inhibition in ELISA.
Compound Unmethylated Methylated Indole-3-acetic acid <0.01 100 Indole-3-acetyl-L-aspartic acid 0.59 2.4 Indole-3-acetyl-L-alanine 2.86 7.35 Indole-3-acetyl-L-phenylalanine 2.15 4 Indole <0.01 ND Indole-3-methanol 2.86 ND 5-Nitroindole <0.01 ND Indole-3-aldehyde <0.01 ND Indole-3-acetamide 3.72 ND Indole-3-acetonitrile 0.97 ND Indole-3-acrylic acid <0.01 <0.01 Indole-3-propionic acid <0.01 4.0 Indole-3-pyruvic acid 1.75 7.69 Indole-3-lactic acid <0.01 <0.01 Indole-3-glyoxylic acid <0.82 2.14 Indole-3-butyric acid 0.82 2.14 Tryptophan <0.01 ND Indole-3-ethanol 3.71 ND Tryptamine <0.01 ND N-Acetyl-D,L-tryptophan <0.01 ND D,L-alpha-methyltryptophan <0.01 ND 4-Chloroindole-3-acetic acid <0.01 40 Indole-3-acetyl-L-leucine <0.01 2.73 Indole-3-acetyl-L-isoleucine <0.01 3.06 Indole-3-carboxylic acid <0.01 <0.01 Indole-5-carboxylic acid <0.01 <0.01 Indole-2-carboxylic acid <0.01 <0.01 Indole3-acetyl-L-glycine 9.1 10.34 indole-3-acetyl-L-valine 0.6 4 5-Hydroxyindole-3-acetic acid <0.01 <0.01 alpha-Naphtylacetic acid 1.9 15.8 2,4-Dichlorophenoxyacetic acid <0.01 <0.01 Processing of plant extracts required prior ELISA analysis may vary from plant to plant. In most cases removal of pigments and lyophilic material by C18 reversed phase chromatography and subsequently by combined DEAE cellulose-reversed phase C18 columns is required. For estimation for IAA samples have to be methylated by diazomethane before ELISA analysis is performed.
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