APR10735 | Anti-IL-6Ra / CD126 Reference Antibody (tocilizumab) Clinisciences

Anti-IL-6Ra / CD126 Reference Antibody (tocilizumab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%
The purity of Anti-IL-6Ra / CD126 Reference Antibody (tocilizumab)is more than 95% ,determined by SEC-HPLC.
Human IL-6R CHOs cells were stained with Anti-IL-6Ra / CD126 Reference Antibody (tocilizumab) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC801=0.4525 nM
Anti-IL-6Ra / CD126 Reference Antibody (tocilizumab)-induced FACS Blocking activity was evaluated using Human IL-6R CHO cells. The IC50 was approximately 1.303nM.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immuno electron microscopy EIA=Enzyme Immunoassay	FC, E, FTA
Primary Accession	P08887
Reactivity	Cynomolgus, Human
Clonality	Monoclonal
Isotype	IgG1
Calculated MW	145.2 KDa

Additional Information
Target/Specificity	IL-6Ra / CD126
Endotoxin	< 0.001EU/ µg,determined by LAL method.
Conjugation	Unconjugated
Expression system	CHO Cell
Format	Purified monoclonal antibody supplied in PBS, pH6.0, without preservative.This antibody is purified through a protein A column.

Protein Information
Name	IL6R (HGNC:6019)
Function	Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal (PubMed:28265003). Signal activation necessitate an association with IL6ST. Activation leads to the regulation of the immune response, acute-phase reactions and hematopoiesis (PubMed:30995492, PubMed:31235509). The interaction with membrane-bound IL6R and IL6ST stimulates 'classic signaling', the restricted expression of the IL6R limits classic IL6 signaling to only a few tissues such as the liver and some cells of the immune system. Whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans- signaling'. Alternatively, 'cluster signaling' occurs when membrane- bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells (Probable).
Cellular Location	[Isoform 1]: Cell membrane {ECO:0000250\|UniProtKB:P22272}; Single-pass type I membrane protein [Soluble interleukin-6 receptor subunit alpha]: Secreted
Tissue Location	[Isoform 2]: Expressed in peripheral blood mononuclear cells and weakly found in urine and serum. 1%-20% of the total sIL6R in plasma is generated by alternative splicing (PubMed:28060820).

Research Areas

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