Anti-IL6 antibody (30-212)

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:500-1:1000 IF/ICC 1:50-1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	IL6
Gene ID:	3569
Uniprot ID:	IL6_HUMAN
Immunogen Region:	30-212
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 30-212 of human IL6 (NP_000591.1).
Immunogen Sequence:	VPPGEDSKDVAAPHRQPLTS SERIDKQIRYILDGISALRK ETCNKSNMCESSKEALAENN LNLPKMAEKDGCFQSGFNEE TCLVKIITGLLEFEVYLEYL QNRFESSEEQARAVQMSTKV LIQFLQKKAKNLDAITTPDP TTNASLLTKLQAQNQWLQDM TTHLILRSFKEFLQSSLRAL RQM

Tissue Specificity	Produced by skeletal muscle.
Post Translational Modifications	N- and O-glycosylated.
Function	Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway (Probable). The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells (Probable). IL6 is a potent inducer of the acute phase response. Rapid production of IL6 contributes to host defense during infection and tissue injury, but excessive IL6 synthesis is involved in disease pathology. In the innate immune response, is synthesized by myeloid cells, such as macrophages and dendritic cells, upon recognition of pathogens through toll-like receptors (TLRs) at the site of infection or tissue injury (Probable). In the adaptive immune response, is required for the differentiation of B cells into immunoglobulin-secreting cells. Plays a major role in the differentiation of CD4(+) T cell subsets. Essential factor for the development of T follicular helper (Tfh) cells that are required for the induction of germinal-center formation. Required to drive naive CD4(+) T cells to the Th17 lineage. Also required for proliferation of myeloma cells and the survival of plasmablast cells. Acts as an essential factor in bone homeostasis and on vessels directly or indirectly by induction of VEGF, resulting in increased angiogenesis activity and vascular permeability. Induces, through 'trans-signaling' and synergistically with IL1B and TNF, the production of VEGF. Involved in metabolic controls, is discharged into the bloodstream after muscle contraction increasing lipolysis and improving insulin resistance. 'Trans-signaling' in central nervous system also regulates energy and glucose homeostasis. Mediates, through GLP-1, crosstalk between insulin-sensitive tissues, intestinal L cells and pancreatic islets to adapt to changes in insulin demand. Also acts as a myokine (Probable). Plays a protective role during liver injury, being required for maintenance of tissue regeneration. Also has a pivotal role in iron metabolism by regulating HAMP/hepcidin expression upon inflammation or bacterial infection. Through activation of IL6ST-YAP-NOTCH pathway, induces inflammation-induced epithelial regeneration.
Protein Name	Interleukin-6 Il-6 B-Cell Stimulatory Factor 2 Bsf-2 Ctl Differentiation Factor Cdf Hybridoma Growth Factor Interferon Beta-2 Ifn-Beta-2
Database Links	Reactome: R-HSA-1059683 Reactome: R-HSA-110056 Reactome: R-HSA-112411 Reactome: R-HSA-2559582 Reactome: R-HSA-381426 Reactome: R-HSA-6783783 Reactome: R-HSA-6785807 Reactome: R-HSA-8853884 Reactome: R-HSA-8957275 Reactome: R-HSA-9660821 Reactome: R-HSA-9662834
Cellular Localisation	Secreted
Alternative Antibody Names	Anti-Interleukin-6 antibody Anti-Il-6 antibody Anti-B-Cell Stimulatory Factor 2 antibody Anti-Bsf-2 antibody Anti-Ctl Differentiation Factor antibody Anti-Cdf antibody Anti-Hybridoma Growth Factor antibody Anti-Interferon Beta-2 antibody Anti-Ifn-Beta-2 antibody Anti-IL6 antibody Anti-IFNB2 antibody

Information sourced from Uniprot.org

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