Anti-MBP antibody (161-304) (Clone: S5MR)

Clonality:	Monoclonal
Clone ID:	S5MR
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:500-1:2000 IF/ICC 1:50-1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	MBP
Gene ID:	4155
Uniprot ID:	MBP_HUMAN
Immunogen Region:	161-304
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 161-304 of human Myelin Basic Protein (P02686).
Immunogen Sequence:	GFLPRHRDTGILDSIGRFFG GDRGAPKRGSGKDSHHPART AHYGSLPQKSHGRTQDENPV VHFFKNIVTPRTPPPSQGKG RGLSLSRFSWGAEGQRPGFG YGGRASDYKSAHKGFKGVDA QGTLSKIFKLGGRDSRSGSP MARR

Tissue Specificity	MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
Post Translational Modifications	Several charge isomers of MBP.C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form).are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic. The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6). Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated. Proteolytically cleaved in B cell lysosomes by cathepsin CTSG which degrades the major immunogenic MBP epitope and prevents the activation of MBP-specific autoreactive T cells. Phosphorylated by TAOK2, VRK2, MAPK11, MAPK12, MAPK14 and MINK1.
Function	The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
Protein Name	Myelin Basic Protein Mbp Myelin A1 Protein Myelin Membrane Encephalitogenic Protein
Database Links	Reactome: R-HSA-9619665
Cellular Localisation	Myelin Membrane Peripheral Membrane Protein Cytoplasmic Side Cytoplasmic Side Of Myelin Isoform 3: Nucleus Targeted To Nucleus In Oligodendrocytes
Alternative Antibody Names	Anti-Myelin Basic Protein antibody Anti-Mbp antibody Anti-Myelin A1 Protein antibody Anti-Myelin Membrane Encephalitogenic Protein antibody Anti-MBP antibody

Information sourced from Uniprot.org

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