Anti-MOUSE IgG (H&L) Pre-adsorbed Secondary Antibody

Cat# ASR1626

Size : 1mg

Brand : Abcepta

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Product Information
Description Anti-MOUSE IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins)
Host Donkey
Conjugate Unconjugated
Target Species Mouse
Reactivity Mouse
Clonality Polyclonal
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E, IC
Application Note ELISA 1:20,000-1:100,000
Western Blot 1:2,000-1:10,000
Immunochemistry 1:1,000-1:5,000
Physical State Liquid (sterile filtered)
Host Isotype IgG
Target Isotype IgG (H&L)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Anti-Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in donkey.
Stabilizer None
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Wet Ice
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Mouse IgG and Mouse Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.  Specificity was confirmed using ELISA at less than 1.0% cross reactivity against IgG proteins purified from the species listed above.
Storage Condition Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.