Anti-RABBIT IgG [H&L] (GOAT) Antibody CY5 Conjugated Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins
Cat# 611-110-122
Size : 1mg
Brand : Rockland Immunochemicals
Contact local distributor :
Phone : +1 850 650 7790
Specifications for Rabbit IgG (H&L) Antibody CY5 Conjugated Pre-adsorbed
Product Details
Goat Anti-Rabbit IgG (H&L) Antibody CY5 Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) - 611-110-122
Goat Anti Rabbit IgG Antibody CY5 Conjugation, Goat Anti-Rabbit IgG CY5 Conjugated Antibody
Goat
IgG (H&L)
Cy5™
Polyclonal
IgG
Target Details
Rabbit
Rabbit IgG whole molecule
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
Application Details
Dot Blot, ELISA, WB
IF, Microarray, Other - View References
Anti-Rabbit IgG Antibody CY5 has been tested by ELISA, dot blot, and western blot and is designed for flow cytometry, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Formulation
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Background
Anti-Rabbit IgG Antibody CY5 generated in goat detects rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rabbit IgG (H&L) is conjugated to CY5.
References (3)
Zhu Y et al. (2023). Sorbitol reduction via govorestat ameliorates synaptic dysfunction and neurodegeneration in sorbitol dehydrogenase deficiency. JCI Insight.
Applications
IF, Confocal Microscopy PubMed
Tao, X et al. (2022). Phenylbutyrate modulates polyamine acetylase and ameliorates Snyder-Robinson syndrome in a Drosophila model and patient cells. Jci Insight
Applications
WB, IB, PCA PubMed
Piret G et al. (2010). Chips from chips: Application to the study of antibody responses to methylated proteins. J Proteome Res.
Applications
IF, Confocal Microscopy; Microarray, Microarray chip PubMed
Related Protocols
Adherent Cell Lysis Protocol
ELISA Protocol
Fluorescent Western Blotting Protocol
General Immunoblotting Protocol
Histone Immunoblotting Protocol
Immunocytochemistry (ICC) Protocol
Immunofluorescence (IF) Protocol
In-Cell Western (ICW) Protocol
IP-WB with TrueBlot® Protocol
Multi-Lysate Western Blotting Protocol
Nuclear & Cytoplasmic Extract Protocol
Sandwich ELISA Protocol for Collagen
Suspension Cultured Cell Lysis Protocol
Western Blotting (WB) Protocol
Certificate of Analysis Lookup
Disclaimer
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Related products to: Rabbit IgG (H&L) Antibody CY5 Conjugated Pre-adsorbed
Rabbit IgG (H&L) Secondary Antibody Fluorescein… 611-1202 Rabbit IgG (H&L) Antibody 611-6102 Rabbit IgG Fc Antibody Alkaline Phosphatase Conjugated… 611-1523 Rabbit IgG Fc Antibody DyLight™ 549 Conjugated 611-142-003 Rabbit IgG (H&L) Antibody DyLight™ 800 Conjugated 611-145-002 Related Resources
Tips for Selecting the Best Secondary Antibody
Technical Tips
View Technical Tip