ASR4159 | Anti-SUMO (MOUSE) Monoclonal Antibody Clinisciences

Western blot of ySUMO fusion protein. Anti-ySUMO antibody, generated by immunization with recombinant yeast SUMO, was tested by western blot against a SUMO-GFP fusion protein (lane 2). While the actual molecular weight of the fusion protein is 39 kDa, the protein migrates as a 49 kDa band (arrowhead). No reactivity is seen for lane 1 which contains His-tagged GFP protein. The membrane was blocked using BLOTTO. Primary antibody was used at a 1:1,000 dilution in BLOTTO. The membrane was washed and reacted with a 1:10,000 dilution of IRDye® 800 Conjugated Affinity Purified Goat-anti-Mouse IgG (H&L) MX10 (800 nm channel). Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (lane M, 700 nm channel). Other detection systems will yield similar results.

Product Information
Host	Mouse
Conjugate	Unconjugated
Target Species	Yeast
Clonality	Monoclonal
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immuno electron microscopy EIA=Enzyme Immunoassay	WB, IHC, E, I, LCI
Application Note	This monoclonal antibody reacts with yeast SUMO (Smt3) tested by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. Using the specified conditions, this antibody may recognize other prominent intrinsic bands (UBLs or conjugates). Other intrinsic bands are readily detectable at lower dilutions.
Physical State	Liquid (sterile filtered)
Buffer	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen	This antibody was produced in mice by repeated immunizations with full-length recombinant yeast SUMO protein.
Preservative	0.01% (w/v) Sodium Azide

Additional Information
Gene ID	852122
Other Names	852122
Purity	This product is a monoclonal antibody purified from tissue culture supernatant fluid by Protein A chromatography.
Storage Condition	Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Precautions Note	This product is for research use only and is not intended for therapeutic or diagnostic applications.

Protein Information
Name	SMT3
Function	Not known; suppressor of MIF2 mutations.

Anti-SUMO (MOUSE) Monoclonal Antibody