Anti-TNK2 antibody (250-300) {FITC}

Clonality:	Polyclonal
Conjugation:	FITC
Isotype:	IgG
Purification:	Affinity Purified
Concentration:	0.59-0.68 µg/µl
Dilution Range:	WB: 1:500 ELISA: 1:10, 000 IP: 1:200
Storage Instruction:	Store at-20°C for long term storage. Avoid freeze-thaw cycles.

Gene Symbol:	TNK2
Gene ID:	10188
Uniprot ID:	ACK1_HUMAN
Immunogen Region:	250-300
Immunogen:	Synthetic peptide taken within amino acid region 300-400 on ACK1 protein.

Tissue Specificity	The Tyr-284 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages. It also shows a significant increase in expression in prostate cancers during the progressive stages.
Post Translational Modifications	Autophosphorylation regulates kinase activity. Phosphorylation on Tyr-518 is required for interaction with SRC and is observed during association with clathrin-coated pits. Polyubiquitinated by NEDD4 and NEDD4L. Degradation can be induced by EGF and is lysosome-dependent.
Function	Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. May be involved both in adult synaptic function and plasticity and in brain development. Activates AKT1 by phosphorylating it on 'Tyr-176'. Phosphorylates AR on 'Tyr-267' and 'Tyr-363' thereby promoting its recruitment to androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr-287'. Phosphorylates MCF2, thereby enhancing its activity as a guanine nucleotide exchange factor (GEF) toward Rho family proteins. Contributes to the control of AXL receptor levels. Confers metastatic properties on cancer cells and promotes tumor growth by negatively regulating tumor suppressor such as WWOX and positively regulating pro-survival factors such as AKT1 and AR. Phosphorylates WASP.
Protein Name	Activated Cdc42 Kinase 1 Ack-1 Tyrosine Kinase Non-Receptor Protein 2
Cellular Localisation	Cell Membrane Nucleus Endosome Cell Junction Adherens Junction Cytoplasmic Vesicle Membrane Peripheral Membrane Protein Cytoplasmic Side Cytoplasmic Vesicle Clathrin-Coated Vesicle Membrane Clathrin-Coated Pit Cytoplasm Perinuclear Region Cytosol The Tyr-284 Phosphorylated Form Is Found Both In The Membrane And Nucleus Co-Localizes With Egfr On Endosomes Nuclear Translocation Is Cdc42-Dependent Detected In Long Filamentous Cytosolic Structures Where It Co-Localizes With Ctps1
Alternative Antibody Names	Anti-Activated Cdc42 Kinase 1 antibody Anti-Ack-1 antibody Anti-Tyrosine Kinase Non-Receptor Protein 2 antibody Anti-TNK2 antibody Anti-ACK1 antibody

Information sourced from Uniprot.org

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