Anti-PSD-95 Antibody (K28/43)

Our Anti-PSD 95 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone K28/43. It is KO validated, detects chicken, human, mouse, non-human primate, and rat PSD 95, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Immunofluorescence staining of cultured rat hippocampal neurons with K28/43 (green) and K57/1 (red, Kv4.2).
Electron micrograph of K28/43 hippocampal labelling using a post-embedding immunogold method. Immunoparticles (arrows) are seen in the postsynaptic densities of dendritic spines (s) forming asymmetrical synapses with axon terminals (b). Scale bar = 200 nm. Image courtesy of Rafael Lujan (Universidad de Castilla-La Mancha).
Adult rat brain immunohistochemistry.
Immunoblot against adult rat brain membranes (RBM) and adult mouse hippocampal membranes (MHM) from PSD-95 and -93 knockout (KO) mice probed with K28/43 (left) or K28/86 (right) TC supe. Mouse samples courtesy of Richard Huganir (Johns Hopkins University, Howard Hughes Medical Institute).
Western blot of total homogenate (H) and TIF fraction from rat hippocampal neurons treated with vehicle (CTRL) or NMDA. Image from publication CC-BY-4.0. PMID: 35613587
Immunolabelling of fixed primary rat hippocampal neurons for SENP1 and PSD95. Image from publication CC-BY-4.0. PMID: 35739402
Double staining of GluA1 (red) and PSD-95 (green) in P12 MNTB neurons of control (left) and Nlgn3 KO (right) mice. Image from publication CC-BY-4.0. PMID: 35704570
Western blot analysis of PSD-95 and synaptophysin protein in the hippocampus region of AD mice. Image from publication CC-BY-4.0. PMID: 35701825
Expression levels of ARID1B protein in WT mouse brains at E18, P0, P7, P14, P21, P28, P35, and P42, as determined by immunoblot analysis of whole-brain lysates. PSD-95, an abundant excitatory postsynaptic density protein, is shown for comparison. The full-length blot was divided into three parts for immunoblotting with different antibodies. Similar results were obtained from three independent experiments. Image from publication CC-BY-4.0. PMID: 36030255
Immunolabeling of Synapsin 1 (SYN1) and PSD95-positive cells (green and red respectively) within MAP2+ neurons (white) in ight-week-old human brain cortical organoids (BCO) infected at MOI 2.5 and treated with vehicle (Veh) or 20μM Sofosbuvir (SOF). Image from publication CC-BY-4.0. PMID:36327326
Representative images of HEK293 cells transfected with different combinations of PSD95( cat. 75-028, 1:2000), GluN2A-CTD, WT, and R9A Rph3A. Image from publication CC-BY-4.0. PMID:36693856
Primary cultures of rat hippocampal neurons were treated at DIV9 with either 2 μg/μl sOligo or PBS and analyses were performed at DIV16. Scaffolding protein Rph3A and PSD-95 (cat. 75-028, 1:1000) were evaluated by Western blot in TIF from sOligo- or PBS-neurons. Image from publication CC-BY-4.0. PMID:37009450
Representative confocal images of mouse cortical neurons using DAPI, MAP2, and PSD95 (cat. 75-028, 1:1000) in control conditions (upper images) and treated with DAPT inhibitor (lower images). Image from publication CC-BY-4.0. PMID:37059474
Effects of Ank2 cKO on the localization of Kv7.3 at excitatory and inhibitory synapses in cultured mouse cortical neurons (DIV 17). Note that the colocalization, determined by colocalized areas, between Kv7.3 (green) and PSD-95 (cat. 75-028, 1:1000; red) (an excitatory synapse marker) is not changed, whereas the mean intensity of Kv7.3 at the colocalized area is moderately decreased. Image from publication CC-BY-4.0. PMID:37321992
Immunostaining of Cas9-expressing cortical neuronal cultures transduced with mock lentiviral vectors (control, left), lentiviral vectors carrying gRNAs to delete Pcdh15 (Pcdh15 Δex6, middle), and sPcdh15-expressing vectors (sPcdh15, right) with the neuronal markers PSD95 (cat. 75-028) and MAP2. Upper: PSD95-expressing puncta. Middle: overlay of PSD95 and MAP2. Lower: magnification of middle panel. Image from publication CC-BY-4.0. PMID:37595869
Dissociated hippocampal neurons isolated from P0 Emx1Cre:R26P1 and Emx1Cre;R26P1R203W mice were processed for confocal imaging at DIV18 to detect MAP2 (magenta), HA-tagged PACS1 or PACS1R203W (pseudocolored green), PSD95 (cat. 75-028, 1:250; pseudocolored red) and DAPI (blue). Image from publication CC-BY-4.0. PMID:37848409
DIV18 neurons prepared from WT C57BL/6 mice were processed for confocal imaging to detect endogenous HDAC6 (green), PACS1 (red), and PSD95 (cat. 75-028, 1:250; blue), and the fluorescent signal from the indicated spine (yellow line) was measured (Nikon Elements). Image from publication CC-BY-4.0. PMID:37848409
Immunofluorescences against PSD95 cat. 75-028) and Synaptophysin in wild-type, FL, and FL1-injected mouse neurons. Image from publication CC-BY-4.0. PMID:37957716
Single confocal images of wildtype (top), heterozygote (middle) and mutant (bottom), showing the colocalization of vGlut1+ boutons (blue) and PSD95+ clusters (cat. 75-028ed) onto VIP+ proximal dendrites (white outline) (yellow arrows) at P45. Image from publication CC-BY-4.0. PMID:38201256
Immunofluorescence staining of cultured rat hippocampal neurons with K28/43 (green) and K57/1 (red, Kv4.2).

Chicken, Human, Mouse, Non-Human Primate, Rat
ICC, IHC, IP, WB
Mouse

Data Sheet
KO Validated KO Validated Trial Size Trial Size

SKU: 75-028

Product Specific References for Applications and Species

  • Immunocytochemistry: Chicken | Human | Mouse | Rat
  • Immunohistochemistry: Chicken | Human | Mouse | Non-Human Primate | Rat
  • Immunoprecipitation: Human | Mouse | Rat
  • Microfluidic Assay: Mouse
  • PLA: Mouse
  • STED: Mouse
  • STORM: Mouse
  • Western Blot: Chicken | Human | Mouse | Non-Human Primate | Rat
    • Immunocytochemistry: Chicken
    PMID Dilution Publication
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    Immunocytochemistry: Human
    PMID Dilution Publication
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    Immunocytochemistry: Mouse
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    Immunocytochemistry: Rat
    PMID Dilution Publication
    388190941:200Zhang, X, et al. 2024. Daphnetin protects neurons in an Alzheimer disease mouse model and normal rat neurons by inhibiting BACE1 activity and activating the Nrf2/HO-1 pathway. Journal of Neuropathology and Experimental Neurology, 0.
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    32801157not listedCulotta, L., et al. 2020. SULT4A1 Modulates Synaptic Development and Function by Promoting the Formation of PSD-95/NMDAR Complex. Journal of Neuroscience, 7013-7026.
    32275651not listedWillems, J., et al. 2020. ORANGE: A CRISPR/Cas9-based genome editing toolbox for epitope tagging of endogenous proteins in neurons. PLoS Biology, e3000665.
    320666981:1000Erli, F., et al. 2020. Estradiol reverses excitatory synapse loss in a cellular model of neuropsychiatric disorders. Translational Psychiatry, 16.
    Immunohistochemistry: Chicken
    PMID Dilution Publication
    210315601:1000Wahlin, K.J., et al. 2010. Alternative splicing of neuroligin and its protein distribution in the outer plexiform layer of the chicken retina.. Journal of Comparative Neurology, 4938-62.
    Immunohistochemistry: Human
    PMID Dilution Publication
    383615351:50Majumder, J, et al. 2024. Human induced pluripotent stem cell-derived planar neural organoids assembled on synthetic hydrogels. Journal of tissue engineering, 20417314241230633.
    371954491:100Maroto, AF, et al. 2023. The vestibular calyceal junction is dismantled following subchronic streptomycin in rats and sensory epithelium stress in humans. Archives of toxicology, 1943-1961.
    333770841:500Ryan, S.K., et al. 2020. Protocol for Tri-culture of hiPSC-DerivedNeurons, Astrocytes, and Microglia. Cell Press, 100190.
    Immunohistochemistry: Mouse
    PMID Dilution Publication
    391465031:500Pavešković, M., et al. 2024. Alzheimer's disease risk gene CD2AP is a dose-sensitive determinant of synaptic structure and plasticity. Human Molecular Genetics, .
    391290071:200Chae, S., et al. 2024. The dopamine analogue CA140 alleviates AD pathology, neuroinflammation, and rescues synaptic/cognitive functions by modulating DRD1 signaling or directly binding to Abeta. Journal of Neuroinflammation, 200.
    391280141:500Wang, L., et al. 2024. Amitriptyline protects afferent synapses in the cochlea against excitotoxic trauma in vitro. The FEBS Journal, .
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    387364831:100Matsumoto, Y, et al. 2024. Slitrk4 is required for the development of inhibitory neurons in the fear memory circuit of the lateral amygdala. Frontiers in Molecular Neuroscience, 1386924.
    38552924not listedGillani, RL, et al. 2024. Instability of excitatory synapses in experimental autoimmune encephalomyelitis and the outcome for excitatory circuit inputs to individual cortical neurons. Brain, Behavior, and Immunity, 251-260.
    378484091:250Villar-Pazos, S, et al. 2023. Neural deficits in a mouse model of PACS1 syndrome are corrected with PACS1- or HDAC6-targeting therapy. Nature Communications, 6547.
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    357045701:200Han, Y., et al. 2022. Neuroligin-3 Confines AMPA Receptors into Nanoclusters, Thereby Controlling Synaptic Strength at the Calyx of Held Synapses. Science Advances, eabo4173.
    Immunohistochemistry: Non-Human Primate
    PMID Dilution Publication
    251765251:400Nuhn, J.S., et al. 2014. Developmental localization of adhesion and scaffolding proteins at the cone synapse. Gene expression patterns, 36-50.
    Immunohistochemistry: Rat
    PMID Dilution Publication
    38231472not listedJiang, WR, et al. 2024. A circRNA ceRNA network involved in cognitive dysfunction after chronic cerebral hypoperfusion. Aging, 1161-1181.
    371954491:100Maroto, AF, et al. 2023. The Vestibular Calyceal Junction is Dismantled Following Subchronic Streptomycin in Rats and Sensory Epithelium Stress in Humans. Archives of Toxicology, 1943-1961.
    362296541:150Simonsen, Ø.W., et al. 2022. Retrosplenial and Subicular Inputs Converge on Superficially Projecting Layer V Neurons of Medial Entorhinal Cortex. Brain Structure & Function, 2821-2837.
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    280356730.2ug/mlFasoli, A., et al. 2017. Somatic and neuritic spines on tyrosine hydroxylase-immunopositive cells of rat retina. Journal of Comparative Neurology, 1707-1730.
    274656831:250Miller, M.B., et al. 2017. Alternate promoter usage generates two subpopulations of the neuronal RhoGEF Kalirin-7. Journal of Neurochemistry, 889-902.
    Immunoprecipitation: Human
    PMID Dilution Publication
    38452102not listedKaizuka, T, et al. 2024. FAM81A is a postsynaptic protein that regulates the condensation of postsynaptic proteins via liquid-liquid phase separation. PLoS Biology, e3002006.
    Immunoprecipitation: Mouse
    PMID Dilution Publication
    360404820.5mg/mlBarisano, G., et al. 2022. A "Multi-omics" Analysis of Blood-Brain Barrier and Synaptic Dysfunction in APOE4 Mice. The Journal of Experimental Medicine, .
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    292942435ugPatel, M.V., et al. 2018. A Novel Short Isoform of Cytosolic PSD-95 Interactor (Cypin) Regulates Neuronal Development. Molecular Neurobiology, 6269-6281.
    2702181910ug/mlVicidomini, C., et al. 2017. Pharmacological enhancement of mGlu5 receptors rescues behavioral deficits in SHANK3 knock-out mice. Molecular Psychiatry, 689-702.
    Immunoprecipitation: Rat
    PMID Dilution Publication
    321575755ugOmelchenko, A., et al. 2020. Interaction Between CRIPT and PSD-95 Is Required for Proper Dendritic Arborization in Hippocampal Neurons. Molecular Neurobiology, 2479-2493.
    307470345ugPatel, M.V., et al. 2019. A Role for Postsynaptic Density 95 and Its Binding Partners in Models of Traumatic Brain Injury. Journal of Neurotrauma, 2129-2138.
    294299361ugMatt, L., et al. 2018. α-Actinin Anchors PSD-95 at Postsynaptic Sites. Neuron, 1094-1109.
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    Microfluidic Assay: Mouse
    PMID Dilution Publication
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    PLA: Mouse
    PMID Dilution Publication
    330098911:200Al Abed, A.S., et al. 2020. Age-related impairment of declarative memory: linking memorization of temporal associations to GluN2B redistribution in dorsal CA1. Aging Cell, e13243.
    STED: Mouse
    PMID Dilution Publication
    326164701:200Held, R.G., et al. 2020. Synapse and Active Zone Assembly in the Absence of Presynaptic Ca2+ channels and Ca2+ Entry. Neuron, 667-683.
    STORM: Mouse
    PMID Dilution Publication
    326164701:200Held, R.G., et al. 2020. Synapse and Active Zone Assembly in the Absence of Presynaptic Ca2+ channels and Ca2+ Entry. Neuron, 667-683.
    Western Blot: Chicken
    PMID Dilution Publication
    210315601:1500-1:2000Wahlin KJ, et al. 2010. Alternative splicing of neuroligin and its protein distribution in the outer plexiform layer of the chicken retina.. Journal of Comparative Neurology, 4938-62.
    Western Blot: Human
    PMID Dilution Publication
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    384166401:1500Adams, JW, et al. 2024. Loss of GTF2I promotes neuronal apoptosis and synaptic reduction in human cellular models of neurodevelopment. Cell reports, 113867.
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    371719581:1000Aryal, S, et al. 2023. Deep proteomics identifies shared molecular pathway alterations in synapses of patients with schizophrenia and bipolar disorder and mouse model. Cell reports, 1267.
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    349574781:1000Avansini, S.H., et al. 2021. Junctional instability in neuroepithelium and network hyperexcitability in a focal cortical dysplasia human model. Brain: A Journal of Neurology, .
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    297355561:1000Campbell, M.K., et al. 2018. USP8 Deubiquitinates SHANK3 to Control Synapse Density and SHANK3 Activity-Dependent Protein Levels. Journal of Neuroscience, 5289-5301.
    288792011:1000Hennig, K.M., et al. 2017. WNT/β-Catenin Pathway and Epigenetic Mechanisms Regulate the Pitt-Hopkins Syndrome and Schizophrenia Risk Gene TCF4. Molecular Neuropsychiatry, 53-71.
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    281676731:5000Fu, S.J., et al. 2017. Ubiquitin Ligase RNF138 Promotes Episodic Ataxia Type 2-Associated Aberrant Degradation of Human Ca v 2.1 (P/Q-Type) Calcium Channels. Journal of Neuroscience, 2485-2503.
    Western Blot: Mouse
    PMID Dilution Publication
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Bulk Order Anti-PSD-95 Antibody