Armenian Hamster monoclonal antibody APC/Cy5.5 labled to CD3e

Description: Armenian Hamster monoclonal antibody APC/Cy5.5 labled to CD3e Purification: The antibody was purified by affinity chromatography. Clonality: Monoclonal (clone: 500-A2) Conjugation: APC/Cy5.5 Form: Syrian Hamster IgG. Liquid in PBS, pH 7.3, 0.2% BSA, and 0.02% sodium azide. Dilution: 10 μl / assay Gene Symbol: CD3E Alternative Names: T3E; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3eEntrez Gene (Mouse) : SwissProt (Mouse) : Directions for Use : 1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).Storage/Stability : Shipped and store at 4°C for one year. Do not freeze.