AXIN1 ELISA Kit (Chicken)

Cat# OKEH06692

Size : 96Wells

Brand : Aviva Systems Biology

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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityChicken, Gallus gallus
ApplicationELISA-Detection
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Axin1 ELISA Kit (Chicken) (OKEH06692) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Axin1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Axin1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Axin1 captured in the well.
ELISA Kit Range0.312-20ng/ml
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
ELISA Kit Component
ComponentAmount
Axin1 Microplate96 Wells (12 x 8 Well strips)
Axin1 Lyophilized Standard2 x 20 ng
100X Axin1 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity0.094 ng/mL
SpecificityNatural and recombinant Chicken Axin-1
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolAXIN1
Gene Full Nameaxin 1
Alias SymbolsAXIN;axin-1;AXIN1-RGS;axis inhibition protein 1.
NCBI Gene Id395786
Protein NameO42400
Description of TargetAxin-1
Uniprot IDhttps://www.uniprot.org/uniprot/O42400
Protein Accession #NP_990275
Nucleotide Accession #NM_204944