Reagents and instruments for immunology, cell biology and molecular biology.
 
> Bacterial Protein Lysis Buffer, 1x Stength Solution

Bacterial Protein Lysis Buffer, 1x Stength Solution

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Cat# BSP023-4x25ml

Size : 4x25ml

Brand : Bio Basic

Contact local distributor :


Phone : +1 850 650 7790

Bacterial Protein Lysis Buffer, 1x Stength Solution
Storage: (-15 to -20)C
Product Description: Bacterial Protein Lysis Buffer, 1x Stength Solution: Bacterial Protein Lysis Buffer enables mild extraction of proteins from bacteria (E. coli) without the need for mechanical disruption. The reagent may be used for soluble protein extraction and inclusion body purgation from bacterial cell lysates. The kit is supplied with lysozyme and DNase I to improve extraction efficiency of large (> 60 kDa) molecular weight proteins and proteins expressed in inclusion bodies. Furthermore, the kit is supplied with PMSF and DTT to improve protein sility and integrity. Depending on the particular application, additional components, such as protease inhibitors, salts, phosphatase inhibitors and chelating agents may be added to the buffer (add chelating agents only when using the buffer without enzymes). Soluble protein is suile for immunoprecipitation, immunoassay, SDS-PAGE, and affinity purification. 25ml is sufficient for 20 extractions of 100mg wet weight bacterial sample.
Number of Containers: 1
Refrigeration Requirements: Freezer
Shipping Conditions: ICE
UNSPSC Code: 41116113
UNSPSC Category: Cell Lysis Reagents
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