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Blocking Buffer Sampler Kit

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Cat# orb348636-1

Size : 1Kit

Brand : Biorbyt

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    Blocking Buffer Sampler Kit

    Catalog Number: orb348636

    Catalog Numberorb348636
    CategoryAssays and Kits
    DescriptionBlocking Buffer Sampler Kit
    Tested applicationsELISA, IF, IHC, WB
    Concentrationn/a
    Dilution rangeELISA: User Optimized, IHC: User Optimized, IF: User Optimized, WB: User Optimized
    Form/AppearanceLiquid and Lyophilized
    PurityLyophilized normal goat serum was prepared from normal goat serum by a multi-step process which includes delipidation and selective precipitation. Assay by immunoelectrophoresis resulted in a multiple precipitin arcs against anti-Goat Serum. BSA has Purity (%): 100% by Agarose Zone Electrophoresis Protease: < 0.005 units/mg by Enzymatic Assay. And no detectable IgG by Radial Immunodiffusion. Blocking Buffer (2X) for Fluorescent Western Blotting, ELISA Microwell blocking buffer with stabilizer, and Fish gel concentrate were aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The products were tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and found to be negative for bacteria.
    Hazard InformationNon-Toxic
    StorageDo not freeze kit. Store kit at 4° C. Kit contains multiple components. See kit insert for complete instructions.
    Buffer/PreservativesSee application note.
    Alternative namesBlocking Buffer Sampler Kit, Normal Goat Serum, NG
    NoteFor research use only
    Application notesBlocking Buffer Sampler Kit contains: NORMAL GOAT SERUM (NGS), BOVINE SERUM ALBUMIN - Fraction V (Immunoglobulin and Protease Free), Blocking Buffer (2X) for Fluorescent Western Blotting, ELISA Microwell Blocking Buffer with Stabilizer (Azide and Mercury Free) - Designed for ELISA assays, 10X TBS Fish Gel Concentrate (Azide and Mercury free) is a blocking agent for immunoassays and western blot.
    Expiration DatePlease enquire.
    Blocking Buffer Sampler Kit

    Biorbyt produces a wide variety of buffers and substrates for use in ELISAs. Antigen was diluted in ELISA Microwell Coating Stabilizer (p/n orb348581) added to the microwell plate and incubated overnight at 4°C. The plate was then blocked with ELISA Microwell Blocking Buffer with Stabilizer (p/n orb348584) for 2 hours. The primary antibody was diluted in PBS Fish Gel Concentrate (1:10)(p/n orb348587), added to the plate, and allowed to incubate 1 hour at room temperature. HRP conjugated secondary antibody was diluted in HRP Conjugate Stabilizer, added to the plate, and allowed to incubate for 30 minutes at room temperature. TMB ELISA Peroxidase Substrate (p/n orb348651) was added to the plate and allowed to incubate for 30 minutes at room temperature. The reaction was then stopped with 1M HCl and read at 450nm.