C9orf116 (NM_144654) Human Tagged ORF Clone

CAT#: RG205731

  • TrueORF®

C9orf116 (tGFP-tagged) - Human chromosome 9 open reading frame 116 (C9orf116), transcript variant 2

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro

AAV Particle: DDK


Plasmid Map

Product Images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Symbol C9orf116
Synonyms PIERCE1; RbEST47
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG205731 representing NM_144654
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGAGGAATGCCCCAGAGCGTGCGCGGAGCCTGTGGCGCCCAAGGCCACGGCCCCGCCGGAGAGGA
CCAGCGACTACTACCGCGTGAGCGCGGACCTGCCGGGCAGGTTCAACAACCCGGGGTGGTTCCGGGGCTA
CAGGACCCAGAAGGCTGTCTCCGTGTACAGGACCAGTAACCAGGCTTACGGGAGCAGAGCCCCCACCGTG
CACGAGATGCCTCGGGTGGAATGTTCCGGAACAATACTCTCAATGTTTACCTGGAGAAAAGCATCG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG205731 representing NM_144654
Red=Cloning site Green=Tags(s)

MAEECPRACAEPVAPKATAPPERTSDYYRVSADLPGRFNNPGWFRGYRTQKAVSVYRTSNQAYGSRAPTV
HEMPRVECSGTILSMFTWRKAS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_144654
ORF Size 276 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_144654.3
RefSeq Size 685 bp
RefSeq ORF 279 bp
Locus ID 138162
UniProt ID Q5BN46
Cytogenetics 9q34.3

Other Versions