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> Catenin, beta (FITC)

Catenin, beta (FITC)

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Cat# C2069-50C-FITC-100ul

Size : 100ul

Brand : US Biological

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C2069-50C-FITC Catenin, beta (FITC)

Clone Type
Polyclonal
Host
mouse
Source
chicken
Isotype
IgG1,k
Grade
Affinity Purified
Applications
IF IHC IP WB
Crossreactivity
Ch Hu Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C

Adherens junctions (AJ; also referred to as zonula adherens) are required for both the establishment and maintenance of epithelial layers (1). In addition, these junctions have been identified in several other cell types including cardiac myocytes and fibroblasts (1). AJ subserve several important functions including: mediating intercellular adhesion, sensing the presence of neighboring cells, and anchoring the actin cytoskeleton (1,10). AJ are multiprotein complexes that are assembled around cell adhesion molecules called cadherins. Cadherins are a multifunctional family of Ca2+-dependent, transmembrane glycoproteins which promote cell-cell adhesion (6). The cadherin extracellular domain mediates homophilic interactions between like cadherin molecules on neighboring cells, while the intracellular domain interacts with several cytoplasmic proteins which include: a-catenin, b-catenin, g-catenin (plakoglobin), and the tyrosine kinase substrate p120cas (2,4,7,9,13,16). Cadherin-catenin interactions are required for complete cadherin activity and regulate the interaction between cadherins and the actin-based cytoskeleton (2,4,6,9,13,17). In fact, deletion of the cadherin cytoplasmic domain produces an adhesion-defective molecule that is unable to interact with the cytoskeleton (6,8). ||Applications: |Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.||Recommended Dilution:|Immunofluorescence: 5–10ug/ml |Western Blot: 1ug/ml|Immunoprecipitation: 2–5ug |Immunohistochemistry (Formalin-fixed and paraffin-embedded tissue): 2–10ug/ml. Requires heat induced epitope retrieval. |Optimal dilutions to be determined by the researcher.||Positive Control:|HeLa, A431 and WI-38 human cell lines||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. |Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. ||Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1,k|Clone No: 3G60|Host: mouse|Source: chicken|Concentration: As reported|Form: Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC).|Purity: Purified by Protein A affinity chromatography.|Immunogen: Fusion protein consisting of the maltose binding protein fused to a 100 amino acid segment of the C-terminus of chicken b-Catenin.|Specificity: Recognizes chicken b-Catenin protein (~92kD). Does not crossreact with the related 83kD g-catenin (plakoglobin) protein. Also specifically detects an additional 120kD protein of unknown identity in extracts derived from A431 cells. This band is not detected in lysates derived from HeLa cells or human fibroblasts. Species Crossreactivity: Human & mouse. Reactivity with rat is likely but not confirmed. ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Fusion protein consisting of the maltose binding protein fused to a 100 amino acid segment of the C-terminus of chicken b-Catenin.
Form
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes chicken b-Catenin protein (~92kD). Does not crossreact with the related 83kD g-catenin (plakoglobin) protein. Also specifically detects an additional 120kD protein of unknown identity in extracts derived from A431 cells. This band is not detected in lysates derived from HeLa cells or human fibroblasts. Species Crossreactivity: Human & mouse. Reactivity with rat is likely but not confirmed.
References
1. Geiger, B., et al., Curr. Opin. Cell Biol. 1: 103–109 (1989). 2. Ozawa, M., et al., PNAS USA 87: 4246–4250 (1990). 3. Peifer, M., et al., Cell 63: 1167–1178 (1990). 4. McCrea, P.D., et al., J. Biol. Chem. 266: 4514–4520 (1991). 5. McCrea, P.D., et al., Science 25: 1359–1361 (1991). 6. Takeichi, M., et al., Science 51: 1451–1455 (1991). 7. Knudsen, K.A., et al., J. Cell. Biol. 18: 671–679 (1992). 8. Ozawa, M., et al., J. Cell. Biol. 116: 989–996 (1992). 9. Johnson, K.R., et al., Exp. Cell Res. 207: 252–260 (1993). 10. Peifer, M., et al., Science 262: 1667–1668 (1993). 11. Rubinfeld, B., et al., Science 262: 1731–1734 (1993). 12. Su, L.-K., et al., Science 262: 1734–1737 (1993). 13. Hinck, L., et al., J. Cell. Biol. 125: 1327–1340 (1994). 14. Kowalczk, A.P., et al., J. Biol. Chem. 269: 31,214–31,223 (1994). 15. Peifer, M., et al., Cell 76: 789–791 (1994). 16. Daniel, J.M., et al., Mol. Cell. Biol. 270: 4819–4824 (1995). 17. Knudsen, K.A., et al., J. Biol. Chem. 130: 67–77 (1995). 18. Bornslaeger, E.A., et al., J. Cell Biol. 134: 985–1001 (1996). 19. Yamaoto, M., et al., J. Biol. Chem. 272(19): 12,492–12,494 (1997). 20. Sakanaka, C., et al., Pro. Natl. Acad. Sci. 95: 3020–3023 (1998). 21. Wu, S., et al., PNAS USA 95(25): 14,979–14,984 (1998). 22. Tesco, G., et al., J. Biol. Chem. 273(51): 33,909–33,914 (1998). 23. Li, Y., et al., Mol. Cell. Biol. 18: 7216–7224 (1998).