CD13 (Aminopeptidase N) (PE)

Cat# 227402-100T

Size : 100Tests

Brand : US Biological

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227402 CD13 (Aminopeptidase N) (PE)

Clone Type
Polyclonal
Host
mouse
Source
equine
Isotype
IgG1
Grade
Affinity Purified
Applications
FC
Crossreactivity
Eq
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

Recognizes the equine CD13 cell surface antigen, also known as Aminopeptidase N, a single-pass type II membrane protein belonging to the peptidase N family with a molecular weight of between 150-170kD.|CD13 is widely expressed by a range of cell types including all blood neutrophils, basophils, monocytes, fibroblasts, kidney epithelial cells, endothelial cells and mesenchymal stem cells, but not by T or B cells. It is involved in a broad spectrum of biological processes and is believed to be linked to a number of disease states including tumor invasion (Saiki et al. 1993).||May be used for differentiating myeloid and lymphoid linage cells in tumors of the haematopoietic system.||Applications: |Suitable for use in Flow Cytometry. Other applications not tested.||Recommended Dilution:|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Store product at 4°C before and after reconstitution. DO NOT FREEZE! Lyophilized and reconstituted products are stable for 12 months at 4°C after receipt. Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product centrifuge the original vial prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: CVS19|Host: mouse|Source: equine|Concentration: Not determined |Form: Supplied as a lyophilized powder in PBS, 1% BSA, 5% sucrose, 0.09% sodium azide. Reconstitute with 1ml ddH2O. Labeled with R-Phycoerythrin (PE). |Purity: Purified by Protein G affinity chromatography.|Immunogen: Equine leucocytes.|Specificity: Recognizes equine CD13.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Equine leucocytes.
Form
Supplied as a lyophilized powder in PBS, 1% BSA, 5% sucrose, 0.09% sodium azide. Reconstitute with 1ml ddH2O. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes equine CD13.
References
1. Kydd, J.H. & Antczak, D.F. (1991) Report of the First International Workshop on Equine Leucocyte Antigens, Cambridge, UK, July 1991 Equine Immunology 4: 5. 2. Dunkel, B. et al. (2009) Neutrophil and platelet activation in equine recurrent airway obstruction is associated with increased neutrophil CD13 expression, but not platelet CD41/61 and CD62P or neutrophil-platelet aggregate formation. Vet Immunol Immunopathol. 131: 25-32. 3. Lunn, D.P. et al. (1998) Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995. Vet Immunol Immunopathol. 62: 101-143. 4. Loftus J.P et al. (2006) Matrix metalloproteinase-9 in laminae of black walnut extract treated horses correlates with neutrophil abundance. Vet Immunol Immunopathol. 113:267-76. 5. Aalberts, M. et al. (2012) Spermatozoa recruit prostasomes in response to capacitation induction. Biochim Biophys Acta.1834: 2326-35. 6. Maia, L. et al. (2013) Immunophenotypic, immunocytochemistry, ultrastructural, and cytogenetic characterization of mesenchymal stem cells from equine bone marrow. Microsc Res Tech. 76: 618-24. 7. Radcliffe, C.H. et al. (2010) Temporal analysis of equine bone marrow aspirate during establishment of putative mesenchymal progenitor cell populations. Stem Cells Dev. 19: 269-82.