CD45R antibody [RA3-6B2] (APC)
Cat# GTX00486-07-100ug
Size : 100ug
Brand : GeneTex
Contact local distributor :
Phone : +1 850 650 7790
![Anti-CD45R antibody [RA3-6B2] (APC) used in Flow cytometry (FACS). GTX00486-07](http://www.genetex.com/templates/default/js/lightbox/images/loading.gif "Anti-CD45R antibody [RA3-6B2] (APC) used in Flow cytometry (FACS). GTX00486-07")
Host | Rat |
|---|---|
Clonality | Monoclonal |
Clone Name | RA3-6B2 |
Isotype | IgG2a |
Application | FACS |
Reactivity | Human, Mouse, Cat |
| Package | 100 μg |
Datasheet File View product citations for antibody GTX00486-07 on CiteAb
PRODUCT
Summary
The RA3-6B2 antibody reacts with the human and mouse CD45 isoform known as CD45R, or B220, a protein tyrosine phosphatase of ≥ 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns, all critical for leukocyte function. In mouse, the CD45R/B220 isoform is predominantly found on B cells, at varying levels on all stages from pro-B cells to activated B cells, and may also be detected on certain T cell and NK cell subsets. It is of note that B220 is not similarly expressed on human B cells, where it appears to be differentiation-specific and therefore expressed on only some B cell subsets. Other forms of CD45 with restricted cellular expression include CD45RA, CD45RB, CD45RO and several others.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application | Recommended Dilution |
|---|---|
| FACS | Assay dependent |
Not tested in other applications.
Calculated MW
145 kDa. ( Note )
Product Note
This antibody recognizes an epitope on CD45R, which is expressed at all developmental stages of B cells, including activated B cells, but also on subsets of NK and T cells. T cells detected by this antibody are supposed to be in activated state.
PROPERTIES
Form
Liquid
Buffer
10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
Abelson murine leukemia virus-induced pre-B tumor cells
Purification
Purified by affinity chromatography
From tissue culture supernatant
From tissue culture supernatant
Conjugation
Allophycocyanin (APC) Wavelength
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
protein tyrosine phosphatase, receptor type, C , B220 , CD45R , Cd45 , L-CA , Ly-5 , Lyt-4 , T200 , loc
Cellular Localization
Cell membrane
Database
DATA IMAGES
| | GTX00486-07 FACS Image FACS analysis of C57Bl/6 splenocytes using GTX00486-07 CD45R antibody [RA3-6B2] (APC). |
Clone Reference
2012; A role for GPx3 in activity of normal and leukemia stem cells
Nagashima T et al. Am J Pathol 2011; 178 (1):222-32 Arachidonate 5-lipoxygenase establishes adaptive humoral immunity by controlling primary B cells and their cognate T-cell help.
2011; Loss of Roquin induces early death and immune deregulation but not autoimmunity
2010; Widespread overexpression of epitope-tagged Mdm4 does not accelerate tumor formation in vivo
2005; Dynamic regulation of PU.1 expression in multipotent hematopoietic progenitors
Submit a Reference REVIEW
Clone Reference
2012; A role for GPx3 in activity of normal and leukemia stem cells
Nagashima T et al. Am J Pathol 2011; 178 (1):222-32 Arachidonate 5-lipoxygenase establishes adaptive humoral immunity by controlling primary B cells and their cognate T-cell help.
2011; Loss of Roquin induces early death and immune deregulation but not autoimmunity
2010; Widespread overexpression of epitope-tagged Mdm4 does not accelerate tumor formation in vivo
2005; Dynamic regulation of PU.1 expression in multipotent hematopoietic progenitors
2004; Lupus IgG VH4.34 antibodies bind to a 220-kDa glycoform of CD45/B220 on the surface of human B lymphocytes
1996; Identification of monoclonal antibodies for immunohistochemical staining of feline B lymphocytes in frozen and formalin-fixed paraffin-embedded tissues
1995; Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies
| SDS | |
|---|---|
| PBS.pdf | |
| Sodium Azide.pdf | |