Reagents and instruments for immunology, cell biology and molecular biology.
 
> CD57 (HNK-1, NK (Natural Killer) Cells)

CD57 (HNK-1, NK (Natural Killer) Cells)

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Cat# C2411-32-200ug

Size : 200ug

Brand : US Biological

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C2411-32 CD57 (HNK-1, NK (Natural Killer) Cells)

Clone Type
Polyclonal
Host
mouse
Source
feline
Isotype
IgM
Grade
Affinity Purified
Applications
FC IHC WB
Crossreactivity
Fe Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

CD57 antigen is present on 15-20% of normal peripheral blood mononuclear cells. It is expressed on a subset of natural killer cells (60%) and on a subset of T-lymphocytes. This carbohydrate is also present on N-CAM in the nervous system.||Applications: |Suitable for use in Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested.||Recommended Dilutions:|Immunohistochemistry: Formalin-fixed paraffin embedded tissue sections.|Flow Cytometry: 1ug stains 1x10e6 cells.|Optimal dilutions to be determined by the researcher.||Hybridoma:|P3-NSI/1-Ag4-1 myeloma cells with spleen cells from Balb/c mice||Source: |Tissue culture||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgM|Clone No: 1.BB.500|Host: mouse|Source: feline|Concentration: ~0.1mg/ml|Form: Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% sodium azide.|Purity: Purified by Protein A affinity chromatography.|Immunogen: Feline unfixed cortical homogenate of subfraction area 17.|Specificity: Recognizes a (Mr 110kD) carbohydrate molecule on feline myeloid cells. This surface antigen is associated with myelin-associated glycoprotein (MAG). Blocks binding of HNK-1 (Leu.7) antibody. Species Crossreactivity: human.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Feline unfixed cortical homogenate of subfraction area 17.
Form
Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes a (Mr 110kD) carbohydrate molecule on feline myeloid cells. This surface antigen is associated with myelin-associated glycoprotein (MAG). Blocks binding of HNK-1 (Leu.7) antibody. Species Crossreactivity: human.
References
1. Arimatsu, Y., et al., J. Neuroscience 7: 1250 (1987). 2. Barnstable, C., et al., Brain Research 559: 118 (1995). 3. Abo, T., Balch, C.M., et al., J. Immunol. 127: 1024 (1981).