Cd8a monoclonal antibody, clone 53-6.7 (PE/Cy5.5)
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- More Files
Flow Cytometry
Cells from BALB/c mesenteric lymph nodes were double-stained with Cd8a monoclonal antibody, clone 53-6.7 (FITC) (Cat # MAB6105) and Cd4 monoclonal antibody, clone GK1.5-PE, gated on small lymphocytes and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).
- Specifications
Product Description
Rat monoclonal antibody raised against native Cd8a.
Immunogen
Native purified from mouse thymus/spleen cells.
Host
Rat
Reactivity
Mouse
Specificity
Specificity alpha chain of the murine CD8 heterodimer, Mr 32-34 KDa
Form
Liquid
Conjugation
PE/Cy5.5
Isotype
IgG2a, kappa
Recommend Usage
Flow Cytometry (0.2 ug/106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Immunohistochemistry (Frozen sections)
Flow Cytometry
Cells from BALB/c mesenteric lymph nodes were double-stained with Cd8a monoclonal antibody, clone 53-6.7 (FITC) (Cat # MAB6105) and Cd4 monoclonal antibody, clone GK1.5-PE, gated on small lymphocytes and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA). - Gene Info — Cd8a
- Publication Reference
- Inhibition of CCL7 derived from Mo-MDSCs prevents metastatic progression from latency in colorectal cancer.
Xiaoli Ren, Jianbiao Xiao, Wanning Zhang, Feifei Wang, Yongrong Yan, Xuehui Wu, Zhicheng Zeng, Yumei He, Wei Yang, Wangjun Liao, Yanqing Ding, Li Liang.
Cell Death & Disease 2021 May; 12(5):484.
Application：Flow Cyt, Mouse, Mouse liver, Mouse T cells.
- Extrathymic differentiation of intraepithelial lymphocytes: generation of a separate and unequal T-cell repertoire?
Lefrancois L.
Immunology Today 1991 Dec; 12(12):436.
- Phenotypic complexity of intraepithelial lymphocytes of the small intestine.
Lefrancois L.
Journal of Immunology 1991 Sep; 147(6):1746.
- The Lyt-2, Lyt-3 macromolecules: structural and functional studies.
Ledbetter JA, Seaman WE.
Immunological Reviews 1982 Jan; 68:197.
- Inhibition of CCL7 derived from Mo-MDSCs prevents metastatic progression from latency in colorectal cancer.