Reagents and instruments for immunology, cell biology and molecular biology.
 
> Claudin 1 Polyclonal Antibody

Claudin 1 Polyclonal Antibody

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Cat# E-AB-30939-120

Size : 120uL

Brand : Elabscience

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Phone : +1 850 650 7790

Verified Samples Verified Samples in WB: Hela
Dilution WB 1:500-1:2000,  IHC 1:100-1:300,  IF 1:200-1:1000
Isotype IgG
Host Rabbit
Reactivity Human,  Mouse,  Rat
Applications WB,  IHC-p,  IF
Clonality Polyclonal
Immunogen Synthesized peptide derived from the C-terminal region of human Claudin-1
Synonyms CLD 1,  CLD1,  CLDN 1,  Claudin-1,  Claudin1,  Cldn1,  ILVASC,  SEMP 1,  SEMP1,  Senescence associated epithelial membrane protein,  Senescence associated epithelial membrane protein 1,  Senescence-associated epithelial membrane protein
Swissprot
Calculated MW 23 kDa
Observed MW 22 kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.
Cellular Localization Cell junction, tight junction. Cell membrane.
Concentration 1 mg/mL
Buffer Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol.
Purification Method Affinity purification
Research Areas Cancer,  Signal Transduction
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome.
Cat.No. Product Name Sizes
E-AB-1003 Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated) 500μL , 120μL , 60μL
E-AB-1010 Goat Anti-Rabbit IgG(H+L)(Cyanine3 conjugated) 500μL , 120μL , 60μL
E-AB-1014 Goat Anti-Rabbit IgG(H+L)(FITC conjugated) 500μL , 120μL , 60μL
E-AB-1043 Streptavidin(peroxidase/HRP conjugated) 500μL , 120μL , 60μL
E-AB-1060 Goat Anti-Rabbit IgG(H+L)(Elab Fluor® 594 conjugated) 500μL , 120μL , 60μL
E-AB-1075 Goat Anti-Rabbit IgG(H+L)(Elab Fluor® 647 conjugated) 500μL , 120μL , 60μL
E-AB-1099 Elab Fluor® 405-Goat Anti-Rabbit IgG(H+L) 500μL , 120μL , 1mL
E-AB-1194 HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed 500μL , 120μL , 1mL
E-IR-R217 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution) 50mL , 18mL , 6mL , 3mL
E-IR-R220 Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0) 10mL , 3mL
E-IR-R221 Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0) 10mL , 3mL
E-IR-R304A Western Blot Detection Kit 50Assays
E-IR-R304B High Accuracy and Absorbability Western Blot Detection Kit 50Assays
Other Clones

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Unconjugated

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