Cystatin D (Cystatin 10, Cst10, Carminerin)

Cat# C8950-12-100ug

Size : 100ug

Brand : US Biological

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C8950-12 Cystatin D (Cystatin 10, Cst10, Carminerin)

Clone Type
Polyclonal
Host
sheep
Source
mouse
Isotype
IgG
Grade
Affinity Purified
Applications
WB
Crossreactivity
Mo
Accession #
NP_067380
Shipping Temp
Blue Ice
Storage Temp
-20°C

Cystatin D is a 17kD member of the type 2 cystatin family of proteins. It is expressed in salivary gland, skin, prehypertrophic and hypertrophic chondrocytes. Within the context of endochondral ossification, it may both induce chondrocyte apoptosis and initiate the process of cartilage calcification. Although Cystatin D contains a key cystatin motif (Gln-x-Val-x-Gly), Cystatin D does not appear to inhibit cysteine proteases. Mouse Cystatin D is 148aa in length. It contains one cystatin-like domain aa39-145. There are two potential splice variants. One shows a deletion of aa84-121, while a second shows a Val substitution for aa122-148. Full-length mouse Cystatin D shares 78aa identity with rat Cystatin D. There appears to be no direct human counterpart to mouse Cystatin D, as both human cystatin-C and -D share less that 40% aa identity with mouse Cystatin D.||Applications:|Suitable for use in Western Blot. Other applications not tested.||Recommended Dilution:|Western Blot: 2ug/ml using mouse salivary gland tissue.|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Applications
Product Type: Pab|Isotype: IgG|Host: sheep|Source: mouse|Concentration: ~0.2mg/ml (after reconstitution) |Form: Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with 500ul sterile PBS.|Purity: Purified by immunoaffinity chromatography.|Immunogen: Recombinant protein corresponding to Asn30-Phe148 from mouse Cystatin D, expressed in NSO cells.|Specificity: Recognizes mouse Cystatin D. In direct ELISAs, less than 4% cross-reactivity with recombinant human (rh) Cst1, rhCst2, and rhCst3 is observed.Recombinant human, Cst1, rhCst2, and rhCst3 (4%).||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Recombinant protein corresponding to Asn30-Phe148 from mouse Cystatin D, expressed in NSO cells.
Form
Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with 500ul sterile PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse Cystatin D. In direct ELISAs, less than 4% cross-reactivity with recombinant human (rh) Cst1, rhCst2, and rhCst3 is observed.Recombinant human, Cst1, rhCst2, and rhCst3 (4%).