Cytochrome c Rabbit Polyclonal Antibody

CAT#: AP21247AF-N

Cytochrome c rabbit polyclonal antibody, Azide Free


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Specifications

Product Data
Applications ELISA, ID, IF, IP, R, WB
Recommended Dilution This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of
the customer’s own choice.
Working dilutions in non-precipitating antibody-binding techniques: 1/100-1/4,000.
Reactivities Equine
Host Rabbit
Isotype IgG
Clonality Polyclonal
Immunogen Cytochrome c is isolated and purified from Horse heart.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
Cross-reactivities against enzymes of other sources may occur but have not been determined.
Formulation PBS, pH 7.2 without preservatives and foreign proteins
State: Azide Free
State: Lyophilized IgG fraction
Reconstitution Method Restore by adding 1.0 ml of sterile distilled water
Concentration lot specific
Purification Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Conjugation Unconjugated
Storage Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Stability Shelf life: one year from despatch
Background Cytochrome c is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in the process of oxidative phosphorylation and production of cellular ATP. An increasing amount of interest has been directed toward the role which cytocrome c has been demonstrated to play in apoptotic processes. Following exposure to apoptotic stimuli, cytochrome c is rapidly released from the mitochondria into the cytosol, an event which may be required for the completion of apoptosis in some systems. Cytosolic cytochrome c functions in the activation of caspase-3, and ICE family molecule that is a key effector of apoptosis.
Synonyms CYCS, CYC
Reference Data

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