Dextranase antibody (Peroxidase)
Cat# orb344724-25ul
Size : 25ul
Brand : Biorbyt
Contact local distributor :
Phone : +1 850 650 7790
Item 1 of 2
Item 1 of 2
Dextranase antibody (Peroxidase)
Catalog Number: orb344724
| Catalog Number | orb344724 |
|---|---|
| Category | Antibodies |
| Description | Dextranase antibody (Peroxidase) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ELISA, IP, WB |
| Reactivity | Bacteria |
| Isotype | IgG |
| Immunogen | Dextranase [Penicillium species] |
| Concentration | 1.0 mg/mL |
| Dilution range | ELISA: 1:2,000 - 1:10,000, IP: User Optimized, WB: 1:500 - 1:5,000 |
| Form/Appearance | Liquid (sterile filtered) |
| Purity | Dextranase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Dextranase [Penicillium species]. Cross reactivity against Dextranase from other sources is unknown. |
| Conjugation | HRP |
| UniProt ID | P48845 |
| NCBI | AAB47720.1 |
| Storage | Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
| Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
| Alternative names | rabbit anti-Dextranase Antibody Peroxidase Conjuga |
| Note | For research use only |
| Application notes | Anti-Dextranase Peroxidase has been tested by ELISA and western blot and assayed against 1.0 ug of Dextranase [Penicillium species] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product. |
| Expiration Date | 12 months from date of receipt. |
ELISA Results of Rabbit Anti-Dextranase Antibody Peroxidase Conjugate tested against purified Dextranase. Each well was coated in duplicate with 1.0 µg of Dextranase. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer and TMB substrate (p/n orb348651).
Western Blot of Rabbit Anti-Dextranase Antibody Peroxidase Conjugated. Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: Dextranase. Primary Antibody: Rabbit Anti-Dextranase at 1:1000 overnight at 2-8°C. Secondary Antibody: none. Block: orb348637. Expect MW: ~66 kDa.