DNA (Deoxyribonucleic Acid), Sodium Salt (Calf Thymus)

Cat# D3880-1g

Size : 1g

Brand : US Biological

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D3880 DNA (Deoxyribonucleic Acid), Sodium Salt (Calf Thymus)

Clone Type
Polyclonal
Grade
Molecular Biology Grade
Shipping Temp
Blue Ice
Storage Temp
4°C/-20°C

Specially prepared to be low in protein content but highly polymerized. Suitable as a substrate for deoxyribonuclease. This DNA is an excellent substrate for DNase. A sodium salt, it must be converted by adding magnesium ions to be susceptible to DNase.||• Low protein and RNA content|• Characterized by a high polymerization factor||Source:|Calf thymus isolated by the method established by Marmur.||Form:|Supplied as a highly purified dried powder||Hyperchromicity:|≥27%||Absorbance Ratio (A260/A280):|≥1.8||Recommended Storage Buffer:|10mM Tris-HCl. pH 8 containing 1mM EDTA (TE Buffer)||Quality Control:|SDS-PAGE||Storage and Stability:|Lyophilized powder may be stored at 4°C. Stable for 12 months at 4°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Applications
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Toxicity and Hazards: All products should be handled by qualified personnel only, trained in laboratory procedures.
Form
Supplied as a highly purified dried powder
References
1. Ausubel, F. M., et al., Current Protocols in Molecular Biology, John Wiley (1992). 2. Maniatis, T., et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press (1989)