Donkey anti-Goat IgG (H&L), DyLight® 488 conjugated
Cat# AS101116
Size : 1mg
Brand : Agrisera
Donkey anti-Goat IgG (H&L), DyLight® 488 conjugated
AS10 1116 | Clonality: Polyclonal | Host: Donkey | Reactivity: Goat IgG (H&L)
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Immunogen: | Purified Goat IgG, whole molecule |
Host: | Donkey |
Clonality: | Polyclonal |
Purity: | Immunogen affinity purified donkey IgG. |
Format: | Lyophilized |
Quantity: | 1 mg |
Reconstitution: | For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily |
Storage: | Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
Recommended dilution: | 1 : 20-1 : 2000 for most applications |
![]() Immunofluorescent localization of V-ATPase epsilon subunit of tonoplast H+ATPase in suspension culture of Oryza sativa ssp. japonica cv. 'Unggi 9', using goat anti-V-ATPase, epsilon subunit of tonoplast antibodies (AS09 577A) and donkey anti-Goat IgG (H&L), DyLight® 488 conjugated secondary antibodies (AS10 1116, Agrisera). Vacuolar membrane, tonoplast, is highlighted by yellow arrowheads. DAPI staining of nuclei is pseudocolored red. Courtesy of Dr. Ferhan Ayaydin, Hungarian Centre of Excellence for Molecular Medicine (HCEMM), Szeged, Hungary |
Additional information: | Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative. Based on immunoelectrophoresis, this antibody reacts with:, heavy chains on goat IgG, light chains on all goat immunoglobulinsNo reactivity is observed to, non-immunoglobulin goat serum proteins |
Background: | Donkey anti-Goat IgG (H&L), DyLight®488 Conjugated is a secondary antibody conjugated to DyLight® 488, which binds to Goat IgG (H&L) in immunological assays. DyLight® 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Goat IgG (H&L) DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries. |