K1232-500 | ECL Chemiluminescent Substrate Detection Kit (Ultrasensitive)

Background

ECL Chemiluminescent Substrate Detection Kit (Ultrasensitive) is an enhanced chemiluminescence (ECL) substrate with ultra-high sensitivity and the most sensitive chemiluminescence substrate among our ECL luminescent solutions, achieving low femtogram levels (based on HRP concentration) through HRP. Western blot detection. Its sensitivity, luminous time, and background are noticeably excellent. It has the following characteristics:

(1) Sensitive: Femtogram-grade protein bands can be detected on nitrocellulose or PVDF membranes when appropriate primary and secondary antibodies are used.
(2) Quantification: The quantifiable range of the resulting signal spans two orders of magnitude.
(3) Bright signal: Exposure through film or imaging system for easy image capture.
(4) Long signal duration: Detectable optical signal output up to 8 hours under optimized conditions.
(5) Stabilized reagents: Kit components can be stable at 4°C for one year and at room temperature for 6 months.
(6) Affordable: Formulated to be optimized for very low concentration antibody detection.

When this product substrate is used in conjunction with optimized antibody concentrations and blocking buffers, low-abundance target proteins that cannot be detected by conventional ECL substrates can be detected.

Chemical Properties

Storage	Store the components dry at 4 °C and protect from light
Shipping Condition	Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips	We do not recommend long-term storage for the solution, please use it up soon.

Quality Control

Protocol

Related Biological Data

ECL Chemiluminescent Substrate Detection Kit (Ultrasensitive)

Components and Storage

Components	100 mL	500 mL
ECL Chemiluminescent Substrate Detection Kit (Ultrasensitive)-A	50 mL	250 mL
ECL Chemiluminescent Substrate Detection Kit (Ultrasensitive)-B	50 mL	250 mL
Store the components dry at 4 °C and protect from light for 12 months.

ECL Chemiluminescent Substrate Detection Kit (Ultrasensitive)

Cat# K1232-500

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