ExoBrite™ 490/515 CD9 Flow Antibody (25 tests)

Cat# P003-490-125

Size : 125UL

Brand : Biotium

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ExoBrite™ CD9 Flow Antibody

Validated antibody for optimal detection of EV marker CD9 in purified or bead-bound EVs by flow cytometry.

Technology

Extracellular Vesicle Research
Flow Cytometry Reagents

Tech Tips

Tech Tip: Fluorescent Detection of EVs by Flow Cytometry
Tech Tip: Isolation and Staining of Extracellular Vesicles

The Full Spectrum Blog

Blog: Bringing Light to Extracellular Vesicles

Highlighted Citations

Exosome profiling with CF® dye tetraspanin panel shows single tetraspanin immunocapture methods may limit cancer biomarker detection
High-throughput fluorescence correlation spectroscopy analysis of extracellular vesicle markers

Product Description

ExoBrite™ CD9 Flow Antibody is validated by Biotium for optimal detection of EV marker CD9 in purified or bead-bound EVs by flow cytometry. The antibody conjugates offer exceptional signal-to-noise and include color options for the Pacific Blue™, FITC, PE, and APC channels.

  • NEW: Now with updated buffer formulation for improved signal-to-noise
  • Optimal detection of EV marker CD9 by flow cytometry
  • Validated for purified or bead-bound EVs
  • Bright signal and low background
  • Available in 4 colors

The most common proteins used as EV markers are CD9, CD63, and CD81, members of the tetraspanin family. These tetraspanins are broadly expressed on many cell types and can therefore be detected on many types of EVs, but their expression levels vary depending on the cell type of origin.

EV antibodies you can trust

Other commercially available antibodies for tetraspanin proteins CD9, CD63, and CD81 are generally not validated for isolated EVs and may require tedious optimization for your EV prep and staining protocol. The antibodies and dye labels of ExoBrite™ Flow Antibody Conjugates were carefully selected and validated for robust detection of isolated EVs. In addition, the antibodies are provided in a proprietary buffer formulation for reduced antibody aggregation and brighter EV staining for optimal accuracy and signal-to-noise. ExoBrite™ Isotype Control Flow Antibodies, which have been found to not react with any target in human cells and have the same isotype as the tetraspanin antibodies, are also available as a negative control.

For general EV staining, Biotium’s ExoBrite™ True EV Membrane Stains offer unparalleled coverage of EVs in a sample and address issues of dye aggregation often seen with PKH and other common membrane dyes. Biotium also offers optimized ExoBrite™ EV Surface stains conjugated to cholera toxin B (CTB), wheat germ agglutinin (WGA), and Annexin V. These stains are specially formulated for bright and specific detection of isolated EVs by flow cytometry. These ExoBrite™ EV Surface stains may also be combined with antibody staining, for multi-parameter analysis.

Biotium also offers conjugated ExoBrite™ Western Antibodies against CD9, CD63, and CD81 designed for optimal detection in EV extracts by fluorescent western blot.

Note: In our testing, we have found that ExoBrite™ 490/515 dye may bind to streptavidin coated surfaces or beads if free biotin binding sites are not blocked. We recommend performing a biotin blocking step after binding your biotinylated capture antibody to streptavidin beads or surfaces when using ExoBrite™ 490/515 conjugates. Alternatively, consider using a different ExoBrite™ dye for staining EVs captured on streptavidin beads or surfaces.

ExoBrite™ Flow Antibody Conjugates

Antibody Ex/Em Detection Channel Size Catalog No.
ExoBrite™ 410/450
CD9 Flow Antibody
416/452 nm Pacific Blue ™ 25 tests P003-410-125
100 tests P003-410-500
ExoBrite™ 490/515
CD9 Flow Antibody
490/516 nm FITC 25 tests P003-490-125
100 tests P003-490-500
ExoBrite™ 560/585
CD9 Flow Antibody
562/584 nm PE 25 tests P003-560-125
100 tests P003-560-500
ExoBrite™ 650/665
CD9 Flow Antibody
652/668 nm APC 25 tests P003-650-125
100 tests P003-650-500
ExoBrite™ R-PE
CD9 Flow Antibody
496, 546, 565/578 PE 25 tests P003-RPE-125
100 tests P003-RPE-500
ExoBrite™ 410/450
CD9 (Mouse) Flow Antibody
416/452 nm Pacific Blue ™ 25 tests P018-410-125
100 tests P018-410-500
ExoBrite™ 490/515
CD9 (Mouse) Flow Antibody
490/516 nm FITC 25 tests P018-490-125
100 tests P018-490-500
ExoBrite™ 560/585
CD9 (Mouse) Flow Antibody
562/584 nm PE 25 tests P018-560-125
100 tests P018-560-500
ExoBrite™ 650/665
CD9 (Mouse) Flow Antibody
652/668 nm APC 25 tests P018-650-125
100 tests P018-650-500
ExoBrite™ 410/450
CD63 Flow Antibody
416/452 nm Pacific Blue ™ 25 tests P004-410-125
100 tests P004-410-500
ExoBrite™ 490/515
CD63 Flow Antibody
490/516 nm FITC 25 tests P004-490-125
100 tests P004-490-500
ExoBrite™ 560/585
CD63 Flow Antibody
562/584 nm PE 25 tests P004-560-125
100 tests P004-560-500
ExoBrite™ R-PE
CD63 Flow Antibody
496, 546, 565/578 PE 25 tests P004-RPE-125
100 tests P004-RPE-500
ExoBrite™ 410/450
CD63 (Mouse) Flow Antibody
416/452 nm Pacific Blue ™ 25 tests P022-410-125
100 tests P022-410-500
ExoBrite™ 490/515
CD63 (Mouse) Flow Antibody
490/516 nm FITC 25 tests P022-490-125
100 tests P022-490-500
ExoBrite™ 560/585
CD63 (Mouse) Flow Antibody
562/584 nm PE 25 tests P022-560-125
100 tests P022-560-500
ExoBrite™ 410/450
CD81 Flow Antibody
416/452 nm Pacific Blue ™ 25 tests P005-410-125
100 tests P005-410-500
ExoBrite™ 490/515
CD81 Flow Antibody
490/516 nm FITC 25 tests P005-490-125
100 tests P005-490-500
ExoBrite™ 560/585
CD81 Flow Antibody
562/584 nm PE 25 tests P005-560-125
100 tests P005-560-500
ExoBrite™ R-PE
CD81 Flow Antibody
496, 546, 565/578 PE 25 tests P005-RPE-125
100 tests P005-RPE-500
ExoBrite™ 410/450
CD81 (Mouse/Rat) Flow Antibody
416/452 nm Pacific Blue ™ 25 tests P019-410-125
100 tests P019-410-500
ExoBrite™ 490/515
CD81 (Mouse/Rat) Flow Antibody
490/516 nm FITC 25 tests P019-490-125
100 tests P019-490-500
ExoBrite™ 560/585
CD81 (Mouse/Rat) Flow Antibody
562/584 nm PE 25 tests P019-560-125
100 tests P019-560-500
ExoBrite™ 410/450 IgG1
Isotype Control Flow Antibody
416/452 nm Pacific Blue ™ 25 tests P008-410-125
100 tests P008-410-500
ExoBrite™ 490/515 IgG1
Isotype Control Flow Antibody
490/516 nm FITC 25 tests P008-490-125
100 tests P008-490-500
ExoBrite™ 560/585 IgG1
Isotype Control Flow Antibody
562/584 nm PE 25 tests P008-560-125
100 tests P008-560-500
ExoBrite™ R-PE IgG1
Isotype Control Flow Antibody
496, 546, 565/578 PE 25 tests P008-RPE-125
100 tests P008-RPE-500
ExoBrite™ 650/665 IgG1
Isotype Control Flow Antibody
652/668 nm APC 25 tests P003-650-125
100 tests P003-650-500
 

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