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> Fibronectin(Fn-3), 1mg/mL

Fibronectin(Fn-3), 1mg/mL

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Cat# BNUM2848-50

Size : 50uL

Brand : Biotium

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Phone : +1 850 650 7790

SDS-PAGE Analysis Purified Fibronectin Mouse Monoclonal Antibody (Fn-3). Confirmation of Integrity and Purity of Antibody.
Antibody number

#2848
Antibody reactivity (target)

Fibronectin
Antibody type

Primary
Host species

Mouse
Clonality

Monoclonal
Clone

Fn-3
Isotype

IgG1, kappa
Molecular weight

220 kDa (monomer); 440 kDa (dimer)
Synonyms

Cold insoluble globulin (CIG); FINC; FN1; FNZ; GFND; GFND2; LETS; Migration stimulating factor (MSF); Ugl-Y3
Human gene symbol

FN1
Entrez gene ID

2335
SwissProt

P02751
Unigene

203717
Immunogen

FR5 cells, derived by SV40 transformation of human mammary epithelial cells.
Antibody target cellular localization

Extracellular matrix
Species reactivity

Human
Expected antibody applications

IF (published for clone), WB (published for clone)
Positive control

SW156 cells. Kidney.
Shipping condition

Room temperature
Storage Conditions

Note: store BSA-free antibodies at -10 to -35°C, Store at 2 to 8 °C, Protect fluorescent conjugates from light
Regulatory status

For research use only (RUO)
Antibody/conjugate formulation

Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide
Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended
Product origin

Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot.
Antibody research areas

Cell adhesion, Extracellular matrix
Protocols

PI Protocols for Antibody Based Detection

SDS

SDS Primary Antibodies

Supporting Documents

CF-Dye-Brochure

CF® Dye Quick Reference Flyer

CF® Dyes Absorption and Emission Normalized Data

Download Datasheet

Product Description

Fibronectins are disulfide-linked, dimeric glycoproteins of ~440 kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Epitope of this MAb is located in the 2nd-3rd type-III repeats of fibronectin. Fibronectins are extracellular matrix glycoproteins that are essential for embryonic development. Fibronectins are also involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors. This MAb reacts with human cellular fibronectin, but not plasma fibronectin.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the

CF® Dye Brochure

for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email

info@clinisciences.com

to inquire about stock status and lead times before placing your order.

 Catalog number key for antibody number 2848, Anti-Fibronectin (Fn-3) 

Antibody # prefix Conjugation Ex/Em (nm) Laser line Detection channel Dye Features
BNC04 CF®405S 404/431 405 DAPI (microscopy), AF405

CF®405S Features
BNC88 CF®488A 490/515 488 GFP, FITC

CF®488A Features
BNC68 CF®568 562/583 532, 561 RFP, TRITC

CF®568 Features
BNC94 CF®594 593/614 561 Texas Red®

CF®594 Features
BNC40 CF®640R 642/662 633-640 Cy®5

CF®640R Features
BNC47 CF®647 650/665 633-640 Cy®5

CF®647 Features
BNC74 CF®740 742/767 633-685 775/50

CF®740 Features
BNCB Biotin N/A N/A N/A
BNUB Purified N/A N/A N/A
BNUM Purified,
BSA-free		 N/A N/A N/A
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of Cytiva; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.

References

Note: References for this clone sold by other suppliers may be listed for expected applications.

  1. Am J Physiol Cell Physiol (2002) 282: C654-C664. (IF; WB)
  2. Meth Mol Biol (2009) https://doi.org/10.1007/978-1-59745-413-1_18 (IF)
  3. J Tissue Eng Regen Med. (2020) 14:761-773. (IF, frozen tissue sections)