GART (6A8) Monoclonal Antibody
Cat# bsm-52537R
Size : 100ul
Brand : Bioss
Contact local distributor :
Phone : +1 850 650 7790
GART Recombinant Antibody
Lane 1: Hela Cells; Lane 2: 293T Cells; Lane 3: A431 Cells; Probed with GART (6A8) Monoclonal Antibody (bsm-52537R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with GART (6A8) Monoclonal Antibody (bsm-52537R) at 1:100 in SW480 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse uterus tissue incubated with GART (6A8) Monoclonal Antibody (bsm-52537R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with GART (6A8) Monoclonal Antibody (bsm-52537R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52537r-100ul |
| Product Name | GART Recombinant Antibody |
| Applications | WB, IHC-P, IF(ICC), IHC |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Recombinant Human GART between 200-250 amino acids |
| Clonality | Monoclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 2618 |
| Swiss Prot | P22102 |
| Synonyms | Trifunctional purine biosynthetic protein adenosine-3, Phosphoribosylamine--glycine ligase, Glycinamide ribonucleotide synthetase, Phosphoribosylglycinamide synthetase, Phosphoribosylformylglycinamidine cyclo-ligase, AIR synthase, Phosphoribosyl-aminoimidazole synthetase, Phosphoribosylglycinamide formyltransferase, 5'-phosphoribosylglycinamide transformylase, GAR transformylase, GARS, AIRS, GART |
| Background | Purines are critical for energy metabolism, cell signaling and cell reproduction and also function as precursors for coenzymes, energy transfer molecules, regulatory factors and proteins involved in RNA and DNA synthesis. GART (GAR transformylase), also referred to as AIRS, GARS, PAIS, PGFT, PRGS or GARTF, is 1,010 amino acids in length and is a key folate-dependent trifunctional enzyme with phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase and AICAR (phosphoribosylaminoimidazole synthetase) activity required for de novo purine biosynthesis. Cancer cells require considerable amounts of purines to sustain their accelerated growth and GART is, therefore, a target for cancer chemotherapy. GART is highly conserved in vertebrates. Two isoforms of GART are expressed due to alternative splicing events. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | 1:200-400 |
| IF(ICC) | 1:50-200 |
| IHC | |