The Ghrelin (GHRL) BioAssay™ ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GHRL in rat serum, plasma, tissue homogenates, cell lysates, cell culture superantes and other biological fluids.||Detection Range:|78-5000pg/ml||Sensitivity:|28pg/ml||Intra-Assay CV:|<10%||Inter-Assay CV:|<12%||Kit Components:|*517240A: Microtiter Plate, 1x96 wells, Pre-coated; ready to use|*517240B: Standard, 2x1 vial |517240C: Standard Diluent, 1x20ml|*517240D: Detection Reagent A (green), 1x120ul|*517240E: Detection Reagent B (red), 1x120ul|517240F: Assay Diluent A, 1x12ml|517240G: Assay Diluent B, 1x12ml|517240H: TMB Substrate, 1x9ml|*517240J: Positive Control, 570pg/ml, 1x1vial (Lyophilized)|517240K: Stop Solution, 1x6ml|517240L: Wash Buffer, 30X, 1x20ml||Precaution:|The Stop Solution (517240K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.||Storage and Stability:|Store *517240A, *517240B, *517240D, *517240E and *517240J at -20°C. After reconstitution of *517240J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.||Notes on 365928J: Positive Control||Intended Use:|The positive control is from a rat serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.||Characteristics:|The positive control vial contains rat GHRL in rat serum (lyophilized powder). Note: The rat based serum used in this product are from healthy rat of SPF standard (specific pathogen-free animals).||Expected Values:|570pg/ml||Usage: |Dissolve with 150ul standard diluent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20°C. After reconstitution, store Positive Control vial at 4°C and use within 5 days.||Assay Procedure Summary:|1. Prepare all reagents, samples and standards.|2. Add 100ul standard or sample to each well. Incubate 1 hour at 37°C.|3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour a 37°C.|4. Aspirate and wash 3 times.|5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.|6. Aspirate and wash 5 times.|7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37°C.|8. Add 50ul Stop Solution. Read at 450nm immediately.