Hexokinase (HK) Activity Assay Kit
Cat# E-BC-K610-M-96T
Size : 96T
Brand : Elabscience
Contact local distributor :
Phone : +1 850 650 7790
Detection principle
HK converts glucose to glucose 6-phosphate, which is catalyzed by the enzyme. Meanwhile, NAD+ is reduced to NADH, which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of HK can be calculated by measuring the change of absorbance value at 450 nm.
Performance characteristics
| Synonyms | HK |
| Sample type | animal tissue, cell |
| Sensitivity | 5.93 U/L |
| Detection range | 5.93-40.11 U/L |
| Assay type | Enzyme Activity |
| Assay time | 30 min |
| Precision | Average inter-assay CV: 9.100%Average intra-assay CV: 3.300% |
| Other instruments required | Centrifuge, 37℃ incubator |
| Storage | -20℃ |
| Valid period | 12 months |
Ralated product
| Cat Number | Product Name |
|---|---|
| E-BC-K234-M | Glucose (Glu) Colorimetric Assay Kit (GOD-POD Method) |
| E-BC-K011-M | Glucose-6-phosphate (G6P) Colorimetric Assay Kit |
| E-BC-K056-M | Glucose-6-Phosphate Dehydrogenase (G-6-PD) Activity Assay Kit |
Troubleshooting
Collection and treatment of common samples for metabolism assay detection
Questions and Answers of Metabolism Assay Kits
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