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Western Blot (Cell lysate)
Application

Western Blot (Cell lysate)

Western Blot analysis of HeLa cells with Hif1a monoclonal antibody, clone ESEE122 (ATTO 488) (Cat # MAB18119).

  • Specifications

    Product Description

    Mouse monoclonal antibody raised against partial recombinant mouse Hif1a.

    Immunogen

    Recombinant protein corresponding to amino acids 329-530 of mouse Hif1a.

    Host

    Mouse

    Reactivity

    Human, Mouse

    Form

    Liquid

    Conjugation

    ATTO 488

    Purification

    Protein G purification

    Isotype

    IgG1

    Recommend Usage

    ELISA
    Immunocytochemistry (1:50)
    Immunofluorescence (1:50)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:100)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS, pH 7.4 (50% glycerol, 0.09% sodium azide).

    Storage Instruction

    Store at -20°C.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot (Cell lysate)

    Western Blot analysis of HeLa cells with Hif1a monoclonal antibody, clone ESEE122 (ATTO 488) (Cat # MAB18119).

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Immunocytochemistry

    Immunofluorescence

    Enzyme-linked Immunoabsorbent Assay

  • Gene Info — Hif1a

    Entrez GeneID

    15251

    Protein Accession#

    Q61221

    Gene Name

    Hif1a

    Gene Alias

    AA959795, HIF-1alpha, HIF1alpha, MOP1

    Gene Description

    hypoxia inducible factor 1, alpha subunit

    Gene Ontology

    Hyperlink

    Gene Summary

    O

    Other Designations

    OTTMUSP00000021321