Reagents and instruments for immunology, cell biology and molecular biology.

HIS (Histamine) ELISA Kit

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Cat# E-EL-0032-96T

Size : 96T

Contact local distributor :

Phone : +1 850 650 7790

Brand : Elabscience

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Contact local distributor :

Phone : +1 850 650 7790

Product Summary

Sensitivity	0.94 ng/mL
Detection Range	1.56-100 ng/mL
Sample Volume	50 μL
Total Assay Time	2 h 30 min
Reacitivity	Universal
Specificity	This kit recognizes Universal HIS in samples.No significant cross-reactivity or interference between Universal HIS and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Competitive
Assay Type	Competitive-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal HIS. During the reaction, Universal HIS in the sample or standard competes with a fixed amount of Universal HIS on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal HIS. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal HIS in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Background

In the vertebrate CNS, the histamine levels are regulated by age, sex, and health无disease condition. In humans, histamine deficiency is traditionally associated with narcolepsy, sleep disorders, food intake, and, more recently, schizophrenia and pathological conditions, among which ischemia, traumatic brain无spinal cord injury, Alzheimer’s, Huntington’s, Parkinson’s diseases, amyotrophic lateral sclerosis, and multiple sclerosis Histamine is synthetized intracellularly after the decarboxylation of the amino acid histidine by L-histidine decarboxylase (HDC), an enzyme whose expression is particularly enriched in mammal’s mast cells, basophils, enterochromaffin-like cells, and histaminergic neurons in the posterior hypothalamic area

Research Area

Immunology