A3472-100mg | Hoechst 33342 [875756-97-1] Clinisciences

Description: IC50 Value: N/A Hoechst stains are part of a family of blue fluorescent dyes used to stain DNA. These Bis-benzimides were originally developed by Hoechst AG, which numbered all their compounds so that the dye Hoechst 33342 is the 33342nd compound made by the company. There are three related Hoechst stains: Hoechst 33258, Hoechst 33342, and Hoechst 34580. The dyes Hoechst 33258 and Hoechst 33342 are the ones most commonly used and they have similarexcitation/emission spectra. Both dyes are excited by ultraviolet light at around 350 nm, and both emit blue/cyan fluorescent light around anemission maximum at 461 nm. Unbound dye has its maximum fluorescence emission in the 510-540 nm range. Hoechst dyes are soluble in water and in organic solvents such as dimethyl formamide or dimethyl sulfoxide. Concentrations can be achieved of up to 10 mg/mL. Aqueous solutions are stable at 2-6 °C for at least six months when protected from light. For long-term storage the solutions are instead frozen at ≤-20 °C. The dyes bind to the minor groove of double-stranded DNA with a preference for sequences rich in adenine andthymine. Although the dyes can bind to all nucleic acids, AT-rich double-stranded DNA strands enhance fluorescence considerably. Hoechst dyes are cell-permeable and can bind to DNA in live or fixed cells. Therefore, these stains are often called supravital, which means that cells survive a treatment with these compounds. Cells that express specific ATP-binding cassette transporter proteins can also actively transport these stains out of their cytoplasm. in vitro: N/A in vivo: N/A Clinical trial: N/A

1. Aiyarin Kittilukkana, Jannarong Intakhad, et al. "The Role of Labile Iron on Brain Proteostasis; Could it be an Early Event of Neurodegenerative Disease?." Arch Biochem Biophys. 2024 Jun:756:110020. PMID: 38692471
2. Guolian Bao, et al. "Characterization and immunological effect of outer membrane vesicles from Pasteurella multocida on macrophages." Appl Microbiol Biotechnol. 2024 Feb 26;108(1):238. PMID: 38407600
3. Xian-Da Yang, et al. "Novel nanotherapeutics for cancer immunotherapy by albumin nanoparticles functionalized with PD-1 and PD-L1 aptamers." Cancer Nanotechnology (2024) 15:3.
4. Mei-Qi Zhang, Xin Jia, et al. "Capsaicin functions as a selective degrader of STAT3 to enhance host resistance to viral infection." Acta Pharmacol Sin. 2023 Nov;44(11):2253-2264. PMID: 37311796
5. Tamina Lebek, Mattias Malaguti, et al. "PUFFFIN: A novel, ultra-bright, customisable, single plasmid system for labelling cell neighbourhoods." bioRxiv. September 08, 2023.
6. Zhiduan Cai, Wenjun Luo, et al. "Dual-layer drug release system based on ureteral stents inhibits the formation of ureteral stricture." Chemical Engineering Journal Volume 471, 1 September 2023, 144596.
7. Shuling Wang, Xiaoyu Jiang, et al. "piR-823 inhibits cell apoptosis via modulating mitophagy by binding to PINK1 in colorectal cancer." Cell Death Dis. 2022 May 17;13(5):465. PMID: 35581181
8. Thipjutha Phatruengdet, Piyachat Khuemjun, et al. "Pharmacokinetic/Pharmacodynamic Determinations of Iron-tannic Molecular Nanoparticles with its Implication in MR Imaging and Enhancement of Liver Clearance." Nanotheranostics. 2022 Jan 1;6(2):195-204. PMID: 34976594
9. Yi Zhang, Dandan Zhang, et al. "Effects of RPE‑conditioned medium on the differentiation of hADSCs into RPE cells, and their proliferation and migration." Exp Ther Med. 2017 Oct;14(4):3699-3707. PMID: 29042966

Hide ↑↑

Storage	Store at -20°C
M.Wt	561.93
Cas No.	875756-97-1
Formula	C₂₇H₃₁Cl₃N₆O
Synonyms	bisBenzimide H 33342;HOE 33342;Hoechst-33342;Hoechst33342
Solubility	insoluble in EtOH; ≥28.7 mg/mL in H2O with gentle warming; ≥46 mg/mL in DMSO
Chemical Name	2'-(4-ethoxyphenyl)-6-(4-methylpiperazin-1-yl)-1H,3'H-2,5'-bibenzo01imidazole trihydrochloride
SDF	Download SDF
Canonical SMILES	CCOC1=CC=C(C2=NC3=C(C=C(C4=NC5=C(C=C(N6CCN(C)CC6)C=C5)N4)C=C3)N2)C=C1.Cl.Cl.Cl
Shipping Condition	Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips	We do not recommend long-term storage for the solution, please use it up soon.

Hoechst 33342 [875756-97-1]

Cat# A3472-100mg

Contact local distributor :

Brand : APExBIO Technology

Contact local distributor :

Background

Product Citation

Chemical Properties

Quality Control

You might also be interested by the following products: