Human IL-6 ELISA Kit (96-well)

Cat# EA100030

Size : 1x96wells

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Human IL-6 ELISA Kit (96-well)

SKU
EA100030
Human IL-6 ELISA Kit (96-well)
Specifications
Product Data
ACCN NM_000600
Assay Length 3 hours
Assay Platform 96-well plate
Assay Type Solid Phase Sandwich ELISA
Assay Type Sandwich
Background

Interleukin 6(IL-6) is a multifunctional protein produced by lymphoid and non-lymphoid cells and by normal and transformed cells, including T cells, monocyte/macrophages, fibroblasts, hepatocytes, vascular endothelial cells, cardiac myxomas, bladder cell carcinomas, myelomas, astrogliomas and glioblastomas. The production of IL-6 in these cells is regulated, either positively or negatively, by a variety of signals including mitogens, antigenic stimulation, lipopolysaccharides, IL-1, TNF, PDGF and viruses. For reviews on IL-6, see references 1-5.

The human IL-6 cDNA sequence predicts a protein of 212 amino acid (aa) residues in length with two potential N-glycosylation sites. The hydrophobic N-terminal 28 aa residue signal peptide is cleaved to produce a mature protein of 184 amino acids with four cysteine residues and a predicted molecular mass of 21 kDa . The mouse IL-6 cDNA sequence shows a homology of 42% at the aa level when compared with the human sequence . On the basis of sequence similarity and gene structural motif similarity, IL-6 can be grouped in a family of cytokines that also includes OSM, G-CSF, LIF, and CNTF. All of these cytokines are predicted to have a four helix bundle structure similar to that found for growth hormone, suggesting that they all evolved from a common ancestral gene .

The effects of IL-6 on different cells are numerous and varied. The effect on B cells is stimulation of differentiation and antibody secretion . IL-6 also affects T cells, acting as a co-stimulant with sub-optimal concentrations of PHA or Con A to stimulate IL-2 production and IL-2 receptor expression.

IL-6 exhibits growth factor activity for mature thymic or peripheral T-cells and reportedly enhances the differentiation of cytotoxic T-cells in the presence of IL-2 or IFN-. . IL-6 stimulates production of acute phase proteins by hepatocytes and has colony-stimulating activity on hematopoietic stem cells. IL-6 has growth factor activities and will stimulate the growth of myeloma/hybridoma/ plasmacytoma cells, EBV-transformed B cells, keratinocytes and mesangial cells .

Components Aluminium pouches with a Microwell Plate coated with antibody to human IL-6 (8 x 6)
2 vials human IL-6 Standard lyophilized, 1000 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-human IL-6 antibody
2 vials Streptavidin-HRP solution
1 bottle Standard /sample Diluent
1 bottle Biotin-Conjugate antibody Diluent
1 bottle Streptavidin-HRP Diluent
1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
1 vial Substrate Solution
4 pieces Adhesive Films
package insert
Curve Range 3.9-250pg/ml
figure_legend Representative standard curve for IL-6 ELISA. IL-6 was diluted in serial two-fold steps in Sample Diluent.
Format 48-well strip plate
Gene Symbol IL6
Interference No significant interference observed with available related molecules.
Locus ID 3569
Reactivity Human
Reactivity Human
Reactivity Human
Sample Type Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume 20 uL
Sensitivity 2pg/mL
Signal Colorimetric
Species Human
Specificity Natural and recombinant Human IL-6 Ligand
Standard Curve /assets/images/elisa/kits/128/che0009-096-1-e.jpg
Target Symbol IL6
New No
Description Human IL-6 ELISA Kit (96-well)

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