IgG, Lambda (HRP)

Applications: |Suitable for use in Immunoblot (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry. Other applications not tested.||Recommended Dilutions:|ELISA:1:10,000-1:50,000.|Western Blot: 1:1000-1:5000|Immunohistochemistry: 1:500-1:2500|Optimal dilution determined by the researcher. ||Storage and Stability:|Lyophilized powder is stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C. Reconstituted product is stable for 12 months at -20°C. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.