IHC-plus™ EN1 / Engrailed Antibody (clone 1F5)
Cat# LS-B5401-50
Size : 50ug
Brand : LSBio
Overview
Antibody:
EN1 / Engrailed Mouse anti-Human Monoclonal (1F5) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Specifications
Description
Engrailed antibody LS-B5401 is an unconjugated mouse monoclonal antibody to Engrailed (EN1) from human. It is reactive with human and mouse. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human EN1 / Engrailed
Synonyms
EN1 | Engrailed 1 | Engrailed homolog 1 | Homeobox protein en-1 | Homeobox protein engrailed-1 | Hu-En-1 | Engrailed homeobox 1
Host
Mouse
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
1F5
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Immunogen
EN1 (NP_001417, 266 aa ~ 392 aa) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human Engrailed Homolog 1
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (1:1000)
- ELISA
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot performed with the recombinant protein used as the immunogen.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee About EN1 / Engrailed
Q05925 Q05925
Validation
Anti-EN1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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