Reagents and instruments for immunology, cell biology and molecular biology.

IL-3 (Hematopoietic growth factor, Mast cell growth factor) (HRP)

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Cat# 144739-HRP-100ul

Size : 100ul

Contact local distributor :

Phone : +1 850 650 7790

Brand : US Biological

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Contact local distributor :

Phone : +1 850 650 7790

144739-HRP IL-3 (Hematopoietic growth factor, Mast cell growth factor) (HRP)

Clone Type

Polyclonal

Host

rabbit

Source

human

Swiss Prot

P08700

Isotype

IgG

Grade

Affinity Purified

Applications

E IHC IP N WB

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

-20°C

The gene IL-3 encodes interleukin 3, a hematopoietic colony-stimulating factor (CSF) that is capable of supporting the proliferation of a broad range of hematopoietic cell types.1 Interleukin-3 (IL-3), a protein of 140 amino acids, is chemically synthesized by means of an automated peptide synthesizer and is shown to have the biological activities attributed to native IL-3.2 The cDNA sequence for murine interleukin-3, one of the colony stimulating factors that regulate haematopoiesis, codes for a polypeptide of 166 amino acids including a putative signal peptide.3 The mouse IL 3 gene is located on chromosome 11.4 The human gene encoding IL 3 is tandemly arrayed on the long arm of chromosome 5.5 The standard product used in this kit is recombinant human IL-3, consisting of 133 amino acids with the molecular mass of 15KDa.||UniProt Number:|P08700||Gene ID:|IL3||Applications:|Suitable for use in ELISA, Western Blot, Immunohistochemistry (Paraffin), Neutralization and Immunoprecipitation.||Recommended Dilution:|Optimal dilutions to be determined by the researcher.||Storage and Handling:|Store at -20˚C for one year. After reconstitution, store at 4˚C for one month. Can also be aliquoted and stored frozen at -20˚C for long term.|Avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.||Note: Applications are based on unconjugated antibody.

Applications

Product Type: Pab|Isotype: IgG|Host: rabbit|Source: human|Concentration: As Reported |Form: Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).|Purity: Purified by immunoaffinity chromatography.|Immunogen: E. coli-derived human IL-3 recombinant protein (Position: A20-F152).|Specificity: Recognizes human IL-3. No crossreactivity with other proteins.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

E. coli-derived human IL-3 recombinant protein (Position: A20-F152).

Form

Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).

Purity

Purified by immunoaffinity chromatography.

Specificity

Recognizes human IL-3. No crossreactivity with other proteins.

References

1. Le Beau, M. M.; Epstein, N. D.; O'Brien, S. J.; Nienhuis, A. W.; Yang, Y.-C.; Clark, S. C.; Rowley, J. D. The interleukin 3 gene is located on human chromosome 5 and is deleted in myeloid leukemias with a deletion of 5q. Proc. Nat. Acad. Sci. 84: 5913-5917, 1987.|2. Clark-Lewis, I.; Aebersold, R.; Ziltener, H.; Schrader, J. W.; Hood, L. E.; Kent, S. B. H. Automated chemical synthesis of a protein growth factor for hemopoietic cells, interleukin-3. Science 231: 134-139, 1986.|3. Fung, M. C.; Hapel, A. J.; Ymer, S.; Cohen, D. R.; Johnson, R. M.; Campbell, H. D.; Young, I. G. Molecular cloning of cDNA for murine interleukin-3. Nature 307: 233-237, 1984.|4. Ihle, J. N.; Silver, J.; Kozak, C. A. Genetic mapping of the mouse interleukin 3 gene to chromosome 11. J. Immun. 138: 3051-3054, 1987.|5. Yang, Y.-C.; Kovacic, S.; Kriz, R.; Wolf, S.; Clark, S. C.; Wellems, T. E.; Nienhuis, A.; Epstein, N. The human genes for GM-CSF and IL3 are closely linked in tandem on chromosome 5. Blood 71: 958-961, 1988.