IL-3 (Hematopoietic growth factor, Mast cell growth factor) (MaxLight 490)

Cat# 144739-ML490-100ul

Size : 100ul

Brand : US Biological

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Phone : +1 850 650 7790


144739-ML490 Rabbit Anti-IL-3 (Hematopoietic growth factor, Mast cell growth factor) (MaxLight 490)

Clone Type
Polyclonal
Host
rabbit
Source
human
Swiss Prot
P08700
Isotype
IgG
Grade
Affinity Purified
Applications
FLISA IHC IP N WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

MaxLight™ 490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.||The gene IL-3 encodes interleukin 3, a hematopoietic colony-stimulating factor (CSF) that is capable of supporting the proliferation of a broad range of hematopoietic cell types.1 Interleukin-3 (IL-3), a protein of 140 amino acids, is chemically synthesized by means of an automated peptide synthesizer and is shown to have the biological activities attributed to native IL-3.2 The cDNA sequence for murine interleukin-3, one of the colony stimulating factors that regulate haematopoiesis, codes for a polypeptide of 166 amino acids including a putative signal peptide.3 The mouse IL 3 gene is located on chromosome 11.4 The human gene encoding IL 3 is tandemly arrayed on the long arm of chromosome 5.5 The standard product used in this kit is recombinant human IL-3, consisting of 133 amino acids with the molecular mass of 15KDa.||UniProt Number:|P08700||Gene ID:|IL3||Applications:|Suitable for use in FLISA, Western Blot, Immunohistochemistry (Paraffin), Neutralization and Immunoprecipitation.||Recommended Dilution:|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.||Note: Applications are based on unconjugated antibody.

Applications
Product Type: Pab|Isotype: IgG|Host: rabbit|Source: human|Concentration: As Reported |Form: Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.|Purity: Purified by immunoaffinity chromatography.|Immunogen: E. coli-derived human IL-3 recombinant protein (Position: A20-F152).|Specificity: Recognizes human IL-3. No crossreactivity with other proteins.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
E. coli-derived human IL-3 recombinant protein (Position: A20-F152).
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human IL-3. No crossreactivity with other proteins.
References
1. Le Beau, M. M.; Epstein, N. D.; O'Brien, S. J.; Nienhuis, A. W.; Yang, Y.-C.; Clark, S. C.; Rowley, J. D. The interleukin 3 gene is located on human chromosome 5 and is deleted in myeloid leukemias with a deletion of 5q. Proc. Nat. Acad. Sci. 84: 5913-5917, 1987.|2. Clark-Lewis, I.; Aebersold, R.; Ziltener, H.; Schrader, J. W.; Hood, L. E.; Kent, S. B. H. Automated chemical synthesis of a protein growth factor for hemopoietic cells, interleukin-3. Science 231: 134-139, 1986.|3. Fung, M. C.; Hapel, A. J.; Ymer, S.; Cohen, D. R.; Johnson, R. M.; Campbell, H. D.; Young, I. G. Molecular cloning of cDNA for murine interleukin-3. Nature 307: 233-237, 1984.|4. Ihle, J. N.; Silver, J.; Kozak, C. A. Genetic mapping of the mouse interleukin 3 gene to chromosome 11. J. Immun. 138: 3051-3054, 1987.|5. Yang, Y.-C.; Kovacic, S.; Kriz, R.; Wolf, S.; Clark, S. C.; Wellems, T. E.; Nienhuis, A.; Epstein, N. The human genes for GM-CSF and IL3 are closely linked in tandem on chromosome 5. Blood 71: 958-961, 1988.