IL12B Mouse Monoclonal Antibody [Clone ID: CC301]

Specifications

Product Data
Clone Name	CC301
Applications	ELISA, Elispot, FC, FN, WB
Recommended Dilution	ELISA: 8 µg/ml. This antibody may be used as a capture antibody in a Sandwich ELISA in combination with Biotinylated clone CC326 (Cat.-No AM05876BT-N) as detection reagent (See Bannerman, D.D.et al.) ELISpot. Western Blot: 1/50-1/100. Flow Cytometry: Use 10 µl of 1/10 diluted antibody to label 106 cells in 100 µl. Membrane permeabilisation is required. Functional Assays: 1/100.
Reactivities	Bovine, Human, Sheep
Host	Mouse
Isotype	IgG2a
Clonality	Monoclonal
Immunogen	Recombinant Bovine IL-12. Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Specificity	This antibody recognises the p40 subunit of Bovine Interleukin-12, and binds to both the free subunit and the intact heterodimer. Negative Species: Mouse.
Formulation	PBS State: Low Endotoxin State: Liquid purified IgG fraction from Tissue Culture Supernatant Stabilizer: None Preservative: None
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Database Link	Entrez Gene 281857 Bovine UniProt ID P46282
Background	Interleukin 12 (IL12) can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine activated killer cells, and stimulate the production of IFN gamma by resting PBMC. IL12 induces IFN gamma, TNF alpha production in T and NK cells; costimulation of PBL proliferation; proliferation/differentiation of TH1 T lymphocytes. It is downregulated by IL10; homodimeric p40 antagonistic to functional p70 heterodimer. IL12R beta 1 binds p40 but when dimeric with IL12R beta 2 binds p35.
Synonyms	Interleukin-12B, IL-12B, IL-12 subunit p40, CLMF p40, NKSF2
Reference Data