IL2 Mouse Monoclonal Antibody [Clone ID: 4F12]

Specifications

Product Data
Clone Name	4F12
Applications	ELISA, FN
Recommended Dilution	ELISA: 5-10 µg as capture antibody. Functional Assays: 2-20 µg/ml.
Reactivities	Chicken, Mammalian, Birds
Host	Mouse
Isotype	IgG2a
Clonality	Monoclonal
Immunogen	Recombinant Chicken IL-2.
Specificity	This antibody SM1540A recognises natural and recombinant IL-2 expressed in either E.coli or CHO cells. Clone 4F12 neutralises the proliferative activity of rchIL-2 and recognises a different epitope to Clone 10E7 product code (Cat.-No SM1539A). This antibody can be used as the Neutralising antibody for recombinant Chicken IL-2 bioassays (See Product code SA028).
Formulation	PBS without preservatives State: Azide Free State: Liquid purified IgG fraction Stabilizer: None
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage	Store the antibody undiluted at -20°C. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Gene Name	interleukin 2
Database Link	Entrez Gene 3558 Human UniProt ID P60568
Background	Interleukin 2 (IL2) is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. IL2 has been shown to have antitumor effects in some studies. This is probably mediated by cytotoxic effector cells.
Synonyms	IL-2, TCGF
Reference Data
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	Allograft rejection, Autoimmune thyroid disease, Cytokine-cytokine receptor interaction, Graft-versus-host disease, Jak-STAT signaling pathway, T cell receptor signaling pathway, Type I diabetes mellitus