INS-1E cells
Cat# C0018009
Size : OneFrozenvial
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Disease:
Insulinoma (Isolated clonal INS-1E cells from the parental INS-1 cells) Pancreatic Islets Rattus Norvegicus Pancreas β cells Adherent AddexBio-Optimized RPMI-1640 Medium(C0004-02) + 10% FBS + 0.05 mM 2-mercaptoethanol 1:2 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C Complete culture medium supplemented with 5% (v/v) DMSO I Bacteria: Negative Yeast: Negative Mycoplasma: Negative HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Respond to glucose within the physiological range. Relatively high insulin content. AR-42J cells BxPc-3 cells Capan-1 cells MIA PaCa-2 cells E3LZ10.7 cells A10.7 cells INS-1 cells MIN-6 cells INS-1E cells Origin:
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References for INS-1E cells:
1. Janjic D, Maechler P, et al. (1999). Free radical modulation of insulin release in INS-1 cells exposed to alloxan. Biochem. Pharmacol. 57, 639-648.
2. Andrzejewski D, Brown ML, Ungerleider N, Burnside A, Schneyer AL. Activins A and B Regulate Fate-Determining Gene Expression in Islet Cell Lines and Islet Cells From Male Mice. Endocrinology. 2015 Jul;156(7):2440-50. doi: 10.1210/en.2015-1167. PMID: 25961841
3. Huang Q, Merriman C, Zhang H, Fu D. Coupling of Insulin Secretion and Display of a Granule-resident Zinc Transporter ZnT8 on the Surface of Pancreatic Beta Cells. J Biol Chem. 2017;292(10):4034-4043. PMID: 28130446
4. Carstensen-Kirberg M, Rohrig K, Niersmann C, Ouwens DM, Belgardt BF, Roden M, Herder C. Sfrp5 increases glucose-stimulated insulin secretion in the rat pancreatic beta cell line INS-1E. PLoS One. 2019 Mar 28;14(3):e0213650. PMID: 30921355
5. Plecita-Hlavata L, Engstova H, Jeek J, Holendova B, Tauber J, Petraskova L, Ken V, Jeek P. Potential of Mitochondria-Targeted Antioxidants to Prevent Oxidative Stress in Pancreatic ?-cells. Oxid Med Cell Longev. 2019 May 21;2019:1826303. PMID: 31249641