Reagents and instruments for immunology, cell biology and molecular biology.

IPTG [367-93-1]

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Cat# T4336-1mL

Size : 1mL

Contact local distributor :

Phone : +1 850 650 7790

Brand : TargetMol

Contact local distributor :

Phone : +1 850 650 7790

IPTG

Purity:99.86%

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COA HPLC HNMR

IPTG

Catalog No. T4336Cas No. 367-93-1

IPTG (Isopropyl β-D-thiogalactoside) is a non-metabolizable galactose analog that induces expression of the LAC OPERON.

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Pack Size
500 mg
1 g
5 g
10 g
25 g
1 mL x 10 mM (in DMSO)

Product Introduction

Bioactivity

Description	IPTG (Isopropyl β-D-thiogalactoside) is a non-metabolizable galactose analog that induces expression of the LAC OPERON.
In vitro	At low concentration, IPTG enters cells through lactose permease, but at high concentrations (typically used for protein induction), IPTG can enter the cells independently of lactose permease.
Kinase Assay	For RNA interference (RNAi), A549 cells are plated at 1,500 cells per well in 96-well plates, allowed to adhere for 24 hours, and transfected with 25 nM siRNA oligonucleotide using Dharmafect 4. Transfected cells are treated with the indicated concentrations of GNE-617 (0.1, 1 , 10 , 100 , and 1000 nM) for 72 hours and viability is evaluated with CellTiter-Glo. Lysates for detection of NAPRT1 protein are collected 72 hours after transfection of 1 million A549 cells in 10 cm dishes. For NAPRT1 re-expression, RERF-LC-MS cells are transfected with pCMV6-AC.NAPRT1 and empty vector pCMV6-AC using Amaxa Nucleofector technology and selected with Geneticin
Alias	Isopropyl β-D-thiogalactoside

Chemical Properties

Molecular Weight	238.3
Formula	C9H18O5S
Cas No.	367-93-1

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

Solubility Information

DMSO: 60 mg/mL (251.78 mM)

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	4.1964 mL	20.9820 mL	41.9639 mL	209.8196 mL
5 mM	0.8393 mL	4.1964 mL	8.3928 mL	41.9639 mL
10 mM	0.4196 mL	2.0982 mL	4.1964 mL	20.9820 mL
20 mM	0.2098 mL	1.0491 mL	2.0982 mL	10.4910 mL
50 mM	0.0839 mL	0.4196 mL	0.8393 mL	4.1964 mL
100 mM	0.0420 mL	0.2098 mL	0.4196 mL	2.0982 mL

Calculator

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

mix well and clarify

Dose Conversion

You can also refer to dose conversion for different animals. More

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

inhibitIPTGIsopropyl b-D-thiogalactosideInhibitorIsopropyl beta-D-thiogalactoside