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> IPTG, Dioxane Free (Isopropyl-b-D-thiogalactoside)

IPTG, Dioxane Free (Isopropyl-b-D-thiogalactoside)

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Cat# I8500-5g

Size : 5g

Brand : US Biological

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I8500 IPTG, Dioxane Free (Isopropyl-b-D-thiogalactoside)

Clone Type
Polyclonal
Grade
Molecular Biology Grade
Shipping Temp
RT
Storage Temp
-20°C

IPTG is a carbohydrate used to induce b-galactosidase for the selection of recombinant plasmids. Used to select for lac Y mutants and to induce the lac operon in E. coli. IPTG will also induce the cellular content of lactose permease. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in E. coli.||Synonyms:|IPTG; Isopropyl-ß-D-thiogalactopyranoside; Isopropyl-ß-D-thiogalactoside||CAS No:|367-93-1||Molecular Formula:|C9H18O5S||Molecular Weight:|238.31||Appearance:|White crystalline or powder||Purity: |≥99%||Identity (IR):|Conforms to structure||pH (5%):|5.0-7.0||Solubility:|Colorless, clear, complete||Dioxane:|Not Detected||Specific Rotation (C=1,H2O):|-29° to -35°||Melting Point:|110-114°C||Water (KF):|≤1%||Blue-White Assay:|Induces b-galactosidase in a pUC 18-containing strain.||Storage and Stability:|Stable for 12 months after receipt at -20°C.

Applications
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Toxicity and Hazards: All products should be handled by qualified personnel only, trained in laboratory procedures.
References
US Biological reference:|Bulcha JT, et al. 2019. A Persistence Detector for Metabolic Network Rewiring in an Animal. Cell Rep. 26(2):460-468.e4. doi: 10.1016/j.celrep.2018.12.064|1. Alag, R. et al., (2010) Protein Science 19; 1577–1586. 2. Norgaard, P. & Winther, J.R., Biochem J. 358: 269–274 (2001). 3. Dennis, A.M., et al., Bioconjugate Chem. June 22, epub (2010). 1. Maniatis, et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N. Y., 2nd Ed., 4: 22-8.57 (1987). 4. Wehinger S., et al. Biochim Biophys Acta. 2015 Jan 5;1852(5):693-708. doi: 10.1016/j.bbadis.2014.12.021. [Epub ahead of print].