Isocitrate BioAssay™ Kit (Isocitric Acid)

Isocitrate (Isocitric Acid) is a substrate in the citric acid (TCA) cycle. Isocitrate is formed by the isomerization of citrate catalyzed by the enzyme aconitase. Isocitrate is oxidized by isocitrate dehydrogenase producing alpha-ketoglutarate and generating NADPH. Isocitrate is commonly|found in many fruits and vegetables and their processed products. Industrially, isocitrate is used as a marker to identify the quality and purity of fruit juices. THe isocitrate assay measures the NADPH generated from the oxidation of isocitrate. The NADPH converts the dye to an intense violet color with an absorption maximum at 565nm. The increase in absorbance at 565nm is directly proportional to the isocitrate concentration.||Intended Use:|For quantitative determination of isocitrate and evaluation of drug effects on its metabolism.||Applications:|Isocitrate determination in food, beverage, biological samples (e.g. cell lysate, tissue homogenate, serum, etc.) ||Range:|20-5000um for 10 min reaction.||Detection limit: |20um||Kit Components:|Assay Buffer, 1x10ml|NADP/MTT, 1x1ml|Standard, 1x1ml|Enzyme A, 1x120ul|Enzyme B, 1x120ul||Storage and Stability:|Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.