Reagents and instruments for immunology, cell biology and molecular biology.

Isotype Control, Mouse IgG2a Kappa (MG2a-53), CF647 Conjugate, 0.1 mg/mL

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Cat# BNC471331B-1ML

Size : 1mL

Contact local distributor :

Phone : +1 850 650 7790

Brand : Biotium

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Contact local distributor :

Phone : +1 850 650 7790

Isotype Control, Monoclonal Mouse IgG2a Kappa (MG2a-53)

Product Attributes

Antibody number	#1331B
Antibody reactivity (target)	Isotype Control
Antibody type	Isotype Control
Host species	Mouse
Clonality	Monoclonal
Clone	MG2a-53
Isotype	IgG2a, kappa
Shipping condition	Room temperature
Storage Conditions	Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C
Shelf life	Guaranteed for at least 24 months from date of receipt when stored as recommended
Regulatory status	For research use only (RUO)
Antibody application notes	For coating for ELISA, order Ab without BSA, Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Optimal dilution and staining procedure for a specific application should be determined by user, Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry
Antibody/conjugate formulation	Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide
Product origin	Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot.

Technology

Primary & Secondary Antibody Conjugates

Tech Tips

Protocol: Fluorescent Western Blotting

Protocol: Immunofluorescence Staining of Cells for Microscopy

Tech Tip: Combined Direct and Indirect IF Using Primary Antibodies from the Same Host

Primary antibody formats:

CF® Dye, APC, R-PE, PerCP, biotin, and alkaline phosphatase conjugates: 0.1 mg/mL in PBS, 0.1% BSA, 0.05% sodium azide
Horseradish peroxidase: 0.1 mg/mL in PBS, 0.05% BSA, no sodium azide
Purified, with BSA: 0.2 mg/mL in PBS, 0.05% BSA, 0.05% sodium azide
Purified, BSA-free: 1 mg/mL in PBS, no BSA, no sodium azide

Product Description

This is a non-reactive mouse monoclonal IgG2a kappa antibody labeled with one of our bright and photostable CF® Dyes and other labels. This antibody may serve as a control for a mouse monoclonal IgG2a antibody under identical experimental conditions. Biotium also carries mouse monoclonal isotype control antibodies for IgG1 Kappa (IGG1/1331) and IgG2b Kappa (IGG1/1333).

Isotype control for antibody-based experiments
Available with 6 bright and photostable CF® Dyes and biotin
Suitable for western, flow cytometry, ELISA, immunofluorescence, and immunohistology

CF® Dyes offer exceptional brightness and photostability; learn more about CF® Dyes.

Catalog number key for antibody number 1331B, Anti-Isotype Control (MG2a-53)

Antibody # prefix	Conjugation	Ex/Em (nm)	Laser line	Detection channel	Dye Features
BNC04	CF®405S	404/431	405	DAPI (microscopy), AF405	CF®405S Features
BNC88	CF®488A	490/515	488	GFP, FITC	CF®488A Features
BNC68	CF®568	562/583	532, 561	RFP, TRITC	CF®568 Features
BNC94	CF®594	593/614	561	Texas Red®	CF®594 Features
BNC40	CF®640R	642/662	633-640	Cy®5	CF®640R Features
BNC47	CF®647	650/665	633-640	Cy®5	CF®647 Features
BNC74	CF®740	742/767	633-685	775/50	CF®740 Features
BNCB	Biotin	N/A	N/A	N/A
BNUB	Purified	N/A	N/A	N/A
BNUM	Purified, BSA-free	N/A	N/A	N/A

Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of Cytiva; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.

Note: Listed references are for this antibody clone sold by Biotium and other suppliers.