Application
| WB, IHC, ICC |
|---|---|
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Clonality | Polyclonal |
| Description | Rabbit Anti-KDEL Polyclonal |
| Target/Specificity | Detects KDEL proteins, GRP94, Grp78, PDI and calreticulin. It may also see ERp57 and ERp29. |
| Other Names | Lys Asp Glu Leu Antibody |
| Immunogen | KDEL containing peptide immunogen |
| Purification | Protein A Purified |
| Storage | -20ºC |
| Storage Buffer | PBS pH7.2, 50% glycerol, 0.09% sodium azide |
| Shipping Temperature | Blue Ice or 4ºC |
| Certificate of Analysis | A 1:1000 dilution of SPC-109 was sufficient for detection of KDEL-containing proteins in 20 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-mouse IgG as the secondary. |
| Cellular Localization | Endoplasmic Reticulum |
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Provided below are standard protocols that you may find useful for product applications.
Background
The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). Grp78 and Grp94 and PDI all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER.
References
1. Ozawa K., et al. (2008) Mol Pharmacol. 74:1610.
2. Austin R.C., et al. (2003) J Biol Chem. 278: 17438.