LB Lennox, a nutritionally rich medium, was developed by Lennox for the growth and maintenance of pure cultures of recombinant strains of E. coli. These strains are generally derived from E. coli K12 which are deficient in B vitamin production. LB Broth media formulations have been industry standards for the cultivation of Escherichia coli since the 1950’s (1,2,3). These media have been widely used in molecular microbiology applications for the preparation of plasmid DNA and recombinant proteins (4,5). ||The media are nutrient-rich formulations which provide peptides and peptones, vitamins, and trace elements. The three formulations (Lennox, Miller and Luria) differ in the amount of sodium chloride, thus providing selection of the appropriate osmotic conditions for the particular bacterial strain and desired culture conditions. The low salt formulations, Lennox and Luria, are ideal for cultures requiring salt-sensitive antibiotics such as Zeocin™.||Principles of the Procedure: |Peptides and peptones are provided by Tryptone. Vitamins (including B vitamins) and certain trace elements are provided by Yeast Extract. Sodium ions for transport and osmotic balance are provided by sodium chloride.||BSE-Free: All raw materials of animal origin are sourced from USDA-designated BSE-Free countries.||Dust-Free: Specially milled powder blends are designed to eliminate or greatly reduce airborne particulates.||Homogeneous: Proprietary blending technology ensures complete mixing of components.||Appearance: |Light tan, homogeneous, free flowing powder||Solubility: |Pale yellow to yellow, clear, complete||pH: |7.0±0.2||Companion Products:|Terrific Broth is a richer media that would be suitable if higher cell densities and higher yields for plasmids or protein purifications are necessary.|T2810: Terrific Broth (Powder)|T2810-05: Terrific Broth, Complete with Carbon Source (Powder)|T2810-10: Terrific Broth, Modified for Genomics (Powder)|T2810-11: Terrific Broth, Modified for Fermentation, non-animal (Powder)||L1500: LB Agar Lennox (Powder)|L1505: LB Broth Lennox (Powder)|L1510: LB Top Agar Lennox (Powder)|P9102-40H: Protein Expression Media, LB Broth Lennox (Powder) pEX™|P9102-40H1: Protein Expression Media, LB Broth Lennox (Liquid) pEX™||L1515: LB Agar Miller (Powder)|L1520: LB Broth Miller (Powder)|L1525: LB Top Agar Miller (Powder)|P9102-40G: Protein Expression Media, LB Broth Miller (Powder) pEX™|P9102-40G1: Protein Expression Media, LB Broth Miller (Liquid) pEX™||L1530: L-Broth (Luria Broth) (Powder)