LGI4 (NM_139284) Human Untagged Clone
Cat# SC313909
Size : 10ug
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LGI4 (NM_139284) Human Untagged Clone
| Product Data | |
| Target Symbol | LGI4 |
|---|---|
| Synonyms | AMC1; AMCNMY; LGIL3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | Fully Sequenced ORF >SC313909 representing NM_139284. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGAGGGGCAGGCATTCTGCTGCTGCTGCTGGCTGGGGCGGGGGTGGTGGTGGCCTGGAGACCCCCA AAGGGAAAGTGTCCCCTGCGCTGCTCCTGCTCTAAAGACAGCGCCCTGTGTGAGGGCTCCCCGGACCTG CCCGTCAGCTTCTCTCCGACCCTGCTGTCACTCTCACTCGTCAGGACGGGAGTCACCCAGCTGAAGGCC GGCAGCTTCCTGAGAATTCCGTCTCTGCACCTGCTCCTCTTCACCTCCAACTCCTTCTCCGTGATTGAG GACGATGCATTTGCGGGCCTGTCCCACCTGCAGTACCTCTTCATCGAGGACAATGAGATTGGCTCCATC TCTAAGAATGCCCTCAGAGGACTTCGCTCGCTTACACACCTAAGCCTGGCCAATAACCATCTGGAGACC CTCCCCAGATTCCTGTTCCGAGGCCTGGACACCCTTACTCATGTGGACCTCCGCGGGAACCCGTTCCAG TGTGACTGCCGCGTCCTCTGGCTCCTGCAGTGGATGCCCACCGTGAATGCCAGCGTGGGGACCGGCGCC TGTGCGGGCCCCGCCTCCCTGAGCCACATGCAGCTCCACCACCTCGACCCCAAGACTTTCAAGTGCAGA GCCATAGAGCTGTCCTGGTTCCAGACGGTGGGGGAGTCGGCACTGAGCGTAGAGCCCTTCTCCTACCAA GGGGAGCCTCACATTGTGCTGGCACAGCCCTTCGCCGGCCGCTGCCTGATTCTCTCCTGGGACTACAGC CTGCAGCGCTTCCGGCCCGAGGAAGAGCTGCCCGCGGCCTCCGTGGTGTCCTGCAAGCCACTGGTGCTG GGCCCGAGCCTCTTCGTGCTGGCTGCCCGCCTGTGGGGGGGCTCACAGCTGTGGGCCCGGCCCAGTCCC GGCCTGCGCCTGGCCCCAACGCAGACCCTGGCCCCGCGGCGGCTGCTGCGGCCCAATGACGCCGAGCTC CTGTGGCTGGAAGGGCAACCCTGCTTCGTGGTGGCCGATGCCTCCAAGGCGGGCAGCACCACGCTGCTG TGCCGCGACGGGCCCGGCTTTTACCCGCACCAGAGCCTGCACGCCTGGCACCGGGACACGGACGCTGAG GCCCTGGAGCTGGACGGCCGGCCCCACCTGCTGCTGGCCTCGGCTTCCCAGCGGCCCGTGCTCTTCCAC TGGACCGGTGGCCGCTTCGAGAGACGCACAGACATCCCCGAGGCCGAGGATGTCTATGCCACACGCCAC TTCCAGGCTGGTGGGGACGTGTTCCTGTGCCTCACACGCTACATTGGGGACTCCATGGTCATGCGCTGG GACGGCTCCATGTTTCGTCTGCTGCAGCAACTTCCCTCGCGCGGTGCCCACGTCTTCCAGCCACTGCTC ATCGCCAGGGACCAGCTGGCCATCCTAGGCAGCGACTTCGCCTTCAGCCAGGTCCTCCGCCTTGAGCCT GACAAGGGGCTCCTGGAGCCACTGCAGGAGCTGGGGCCTCCGGCCCTGGTGGCCCCCCGTGCCTTTGCC CACATCACTATGGCCGGCAGACGCTTCCTCTTTGCTGCTTGCTTTAAGGGCCCCACACAGATCTACCAG CATCACGAGATCGACCTCAGTGCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI Plasmid Map |
| ACCN | NM_139284 |
| Insert Size | 1614 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_139284.2 |
| RefSeq Size | 2906 bp |
| RefSeq ORF | 1614 bp |
| Locus ID | 163175 |
| UniProt ID | Q8N135 |
| Cytogenetics | 19q13.11 |
| Domains | EPTP, LRR, LRRCT, LRR_TYP |
| MW | 59.1 kDa |
| Summary | Component of Schwann cell signaling pathway(s) that controls axon segregation and myelin formation (By similarity).[UniProtKB/Swiss-Prot Function] |